Kapron James, Wu Jin, Mauriala Timo, Clark Patsy, Purves Randy W, Bateman Kevin P
Ionalytics, M50-IPF, 1200 Montreal Rd, Ottawa, ON, K1A 0R6 Canada.
Rapid Commun Mass Spectrom. 2006;20(10):1504-10. doi: 10.1002/rcm.2505.
A liquid chromatography/high-field asymmetric waveform ion mobility spectrometry/tandem mass spectrometry (LC-FAIMS-MS/MS) semi-quantitative method was developed for the simultaneous determination of prostaglandin (PG) E2, PGD2, PGF(2alpha), 6-keto-PGF(1alpha), and thromboxane (TX) B2. Diluted samples containing these prostanoids and their tetra-deuterium-substituted internal standards were analyzed by LC followed by either selected reaction monitoring (LC-SRM) or FAIMS and selected reaction monitoring (LC-FRM). FAIMS reduced background noise, separated the isobaric ions PGE2 and PGD2, and separated dynamically interchanging TXB2 anomers. This is the first report of the separation of interconverting anomers by FAIMS. Generally, the LC-FRM chromatograms were more selective than the LC-SRM chromatograms. Its potential was demonstrated by analysis of prostanoids in guinea pig lumbar spinal cord homogenate.
建立了一种液相色谱/高场非对称波形离子淌度光谱/串联质谱（LC-FAIMS-MS/MS）半定量方法，用于同时测定前列腺素（PG）E2、PGD2、PGF(2α)、6-酮-PGF(1α)和血栓素（TX）B2。含有这些前列腺素及其四氘代内标的稀释样品先通过液相色谱分析，然后进行选择反应监测（LC-SRM）或FAIMS与选择反应监测（LC-FRM）。FAIMS降低了背景噪声，分离了等压离子PGE2和PGD2，并分离了动态互换的TXB2异头物。这是首次报道通过FAIMS分离互变异构异头物。一般来说，LC-FRM色谱图比LC-SRM色谱图更具选择性。通过对豚鼠腰脊髓匀浆中前列腺素的分析证明了该方法的潜力。
