Siebers M C, Walboomers X F, Leeuwenburgh S C G, Wolke J G C, Jansen J A
Department of Periodontology and Biomaterials, Radboud University Nijmegen Medical Centre, Nijmegen, PO Box 9101, 6500 HB, The Netherlands.
J Biomed Mater Res A. 2006 Aug;78(2):258-67. doi: 10.1002/jbm.a.30700.
This article describes the influence of the crystallinity of carbonate apatite (CA) coatings on osteoblast-like cell behavior. Porous CA coatings were produced with electrostatic spray deposition (ESD), and subsequently, received heat treatments of 400, 500, or 700 degrees C to induce various coating crystallinities. As a result, an amorphous calcium phosphate (ACP), a crystalline CA (CCA), and a crystalline carbonated hydroxyapatite (CHA) structure were formed, respectively. Uncoated titanium substrates served as the control group. After seeding rat osteoblast-like cells, the initial cell attachment was similar between the groups, and approached 100% after 6 h. Between the various coatings, no differences were observed for proliferation, differentiation, or mineralization. However, proliferation of the osteoblast-like cells was lower on all coated substrates after longer culture periods, compared to the uncoated substrates, while at the same time differentiation was stimulated. Furthermore, after 8 and 16 days of incubation, scanning electron microscopy showed more signs of mineralization on coated substrates, compared to the uncoated substrates. In conclusion, porous ESD-derived CA coatings have a positive effect on the in vitro differentiation of osteoblast-like cells, compared to uncoated, as-machined titanium. However, this effect is not further enhanced by the degree of crystallinity of the ESD-derived CA coatings.
本文描述了碳酸磷灰石（CA）涂层的结晶度对成骨样细胞行为的影响。采用静电喷涂沉积（ESD）制备了多孔CA涂层，随后分别进行400、500或700℃的热处理，以诱导涂层产生不同的结晶度。结果分别形成了无定形磷酸钙（ACP）、结晶性CA（CCA）和结晶性碳酸羟基磷灰石（CHA）结构。未涂层的钛基底作为对照组。接种大鼠成骨样细胞后，各组之间的初始细胞附着情况相似，6小时后接近100%。在不同涂层之间，未观察到增殖、分化或矿化方面的差异。然而，与未涂层的基底相比，在较长培养期后，所有涂层基底上成骨样细胞的增殖均较低，而同时分化受到刺激。此外，在培养8天和16天后，扫描电子显微镜显示，与未涂层的基底相比，涂层基底上有更多矿化迹象。总之，与未涂层的机械加工钛相比，多孔ESD衍生的CA涂层对成骨样细胞的体外分化有积极作用。然而，ESD衍生的CA涂层的结晶度并未进一步增强这种作用。
