Hulshoff J E, van Dijk K, van der Waerden J P, Wolke J G, Ginsel L A, Jansen J A
Department of Oral Function, Dental School, University of Nijmegen, The Netherlands.
J Biomed Mater Res. 1995 Aug;29(8):967-75. doi: 10.1002/jbm.820290808.
A rat bone marrow cell culture was used to evaluate the osteogenic potential of amorphous and crystalline thin calcium phosphate (Ca/P) coatings. The coatings were deposited on titanium discs using a radiofrequency magnetron sputter procedure. Amorphous and crystalline plasma spray Ca/P coated and noncoated titanium discs served as reference material. The cellular behavior was analyzed with quantitative (attachment and proliferation rates) and qualitative (scanning electron microscopy) techniques. No significant differences were found in cell attachment and proliferation rates between the various materials. Scanning electron microscopy showed extracellular matrix formation after 18 days of culture on amorphous plasma-sprayed and the two types of magnetron sputtered coatings. Furthermore, no severe degradation of the magnetron sputtered coatings was observed. They even appeared to induce apatite formation. On basis of the results, we conclude that magnetron sputtering appears to be a promising method to manufacture bioactive ceramic coatings.
采用大鼠骨髓细胞培养来评估非晶态和晶态磷酸钙（Ca/P）薄涂层的成骨潜力。使用射频磁控溅射工艺将涂层沉积在钛盘上。非晶态和晶态等离子体喷涂Ca/P涂层及未涂层的钛盘用作参考材料。采用定量（附着率和增殖率）和定性（扫描电子显微镜）技术分析细胞行为。各种材料之间在细胞附着率和增殖率方面未发现显著差异。扫描电子显微镜显示，在非晶态等离子体喷涂涂层以及两种磁控溅射涂层上培养18天后有细胞外基质形成。此外，未观察到磁控溅射涂层有严重降解。它们甚至似乎能诱导磷灰石形成。基于这些结果，我们得出结论，磁控溅射似乎是制造生物活性陶瓷涂层的一种有前景的方法。
