Izadi H, Subrahmanyam B
Division of Plant Protection, Faculty of Agriculture, Valie Asr University Rafsanjan, Iran.
Commun Agric Appl Biol Sci. 2005;70(4):849-56.
The physiological action of two substituted oxime ethers namely: 4'-(2,6,6-trimethyl-2-cyclohexen-1-yl)-3'-buten-2'(E)-ketoxime-N-O-propylether (compound No. 3) and 4'-(2,6,6-trimethyl-1-cyclohexen-1-yl)-3'-buten-2'(E)-ketoxime-N-O-pentylether (compound No. 34,) were compared with that of JH III in an in vitro assay to monitor the synthesis of RNA and protein in male accessory reproductive gland (MARG) of Spodoptera litura by using 3H-leucine and 3H-uridine, respectively. Both the compounds have stimulated protein synthesis compare to control. Compound No 34 is slightly more effective than JH III in increasing the protein synthesis at physiological concentration of 10(-6) and 10(-5) M. Compound No 3 and JH III have doubled the RNA synthesis and increased the protein synthesis by 1.5 times over the control at 10(-4) to 10(-6) M concentrations. While JH III at 10(-5) M significantly enhanced RNA synthesis, similar effect is produced only at 10(-3) M by compound No 3 and 34.
在体外试验中，使用³H-亮氨酸和³H-尿苷分别监测斜纹夜蛾雄性附属性腺（MARG）中RNA和蛋白质的合成，比较了两种取代肟醚，即4'-（2,6,6-三甲基-2-环己烯-1-基）-3'-丁烯-2'（E）-酮肟-N-O-丙基醚（化合物3号）和4'-（2,6,6-三甲基-1-环己烯-1-基）-3'-丁烯-2'（E）-酮肟-N-O-戊基醚（化合物34号）与保幼激素III（JH III）的生理作用。与对照相比，这两种化合物均刺激了蛋白质合成。在10⁻⁶和10⁻⁵ M的生理浓度下，化合物34号在增加蛋白质合成方面比JH III略有效。在10⁻⁴至10⁻⁶ M浓度下，化合物3号和JH III使RNA合成增加了一倍，蛋白质合成比对照增加了1.5倍。虽然10⁻⁵ M的JH III显著增强了RNA合成，但化合物3号和34号仅在10⁻³ M时产生类似效果。
