Chirgwin Sharon R, Coleman Sharon U, Porthouse Kristina H, Klei Thomas R
Department of Pathobiological Sciences, Louisiana State University School of Veterinary Medicine, Baton Rouge, Louisiana 70803, USA.
J Parasitol. 2006 Feb;92(1):46-51. doi: 10.1645/GE-599R.1.
Infection with mosquito-born filarial nematodes occurs when hosts are bitten by a vector carrying the infective third stage larvae (L3) of the parasites. These larvae, deposited on the skin by the feeding mosquito, are presumed to enter the skin via the vector-induced puncture wound. Larvae of Brugia spp. must then migrate from the entry site, penetrate various skin layers, and locate a lymphatic vessel that leads to their lymphatic predilection site. We have recently established an intradermal (ID) infection model using B. pahangi and the Mongolian gerbil, allowing us to investigate the migratory capability ofB. pahangi. Larval and adult parasites recovered from the peritoneal cavities of gerbils were capable of establishing an infection following ID (larvae) or subcutaneous (adult) injection. Third and fourth stage larvae both migrated away from the injection site within hours, although data suggest they localize to different lymphatic tissues at 3 days postinfection (DPI). Immature adult (28 day) B. pahangi also migrated away from their SC inoculation site within 7 DPI. Mature (45 day) adult B. pahangi displayed little migration away from the SC infection site, suggesting tissue migration may be limited to developing stages of the parasite.
当宿主被携带寄生虫感染性第三期幼虫（L3）的媒介叮咬时，就会发生蚊媒丝状线虫感染。这些由吸血蚊子沉积在皮肤上的幼虫，据推测会通过媒介造成的穿刺伤口进入皮肤。布鲁氏菌属的幼虫随后必须从进入部位迁移，穿透各层皮肤，并找到一条通向其淋巴偏好部位的淋巴管。我们最近利用彭亨布鲁线虫和蒙古沙鼠建立了一种皮内（ID）感染模型，使我们能够研究彭亨布鲁线虫的迁移能力。从沙鼠腹腔中回收的幼虫和成虫在皮内（幼虫）或皮下（成虫）注射后能够建立感染。第三期和第四期幼虫在数小时内都从注射部位迁移开，尽管数据表明它们在感染后3天（DPI）定位到不同的淋巴组织。未成熟成虫（28天）彭亨布鲁线虫在7 DPI内也从皮下接种部位迁移开。成熟（45天）成虫彭亨布鲁线虫从皮下感染部位迁移很少，表明组织迁移可能仅限于寄生虫的发育阶段。
