Kusaba Teruyo, Fujimaki Yasunori, Vincent Albert L, Aoki Yoshiki
Department of Parasitology, Institute of Tropical Medicine, Nagasaki University, 1-12-4 Sakamoto, Nagasaki, 852-8523 Japan.
Parasitol Int. 2008 Jun;57(2):179-84. doi: 10.1016/j.parint.2007.12.006. Epub 2007 Dec 23.
The jird (Mongolian gerbil) is a highly susceptible experimental host for the lymphatic filarial nematode, Brugia pahangi. The chemotactic activity of serum from this host for B. pahangi infective larvae was compared in vitro to that of sera or hemolymph of a wide variety of other organisms including mammals, reptiles, fishes and invertebrates. The range of the Chemotactic Index (CI) was from 96.0 for the jird to 56.2 for a snail. An average of CI of saline control was 4.5. Significant chemotactic activity was present in many organisms, especially mammals, but was not closely related to either the phylogenetic position of the organism and to its known susceptibility as definitive host for B. pahangi. Migratory response was diminished in a consistent way by serial dilution of sera of humans, jirds and fetal bovine serum. Pre-incubation of larvae in fetal bovine serum inhibited migration, especially towards the sera of humans. Inhibition could be reversed by rinsing larvae in saline, longer rinse periods resulting in greater recovery of CI. These results are the first to suggest the activity of the specific amphid chemoreceptors in the chemotaxis of the infective larvae of B. pahangi.
沙鼠（蒙古沙鼠）是淋巴丝虫线虫——彭亨布鲁线虫高度易感的实验宿主。在体外，将该宿主血清对彭亨布鲁线虫感染性幼虫的趋化活性与包括哺乳动物、爬行动物、鱼类和无脊椎动物在内的多种其他生物的血清或血淋巴的趋化活性进行了比较。趋化指数（CI）范围从沙鼠的96.0到蜗牛的56.2。生理盐水对照的CI平均值为4.5。许多生物，尤其是哺乳动物，都存在显著的趋化活性，但这与生物的系统发育位置及其作为彭亨布鲁线虫终末宿主的已知易感性均无密切关系。通过对人、沙鼠和胎牛血清进行系列稀释，迁移反应以一致的方式减弱。将幼虫在胎牛血清中预孵育会抑制迁移，尤其是对人血清的迁移。通过在盐水中冲洗幼虫可逆转抑制作用，冲洗时间越长，CI恢复得越好。这些结果首次表明了特定的两性感觉器化学感受器在彭亨布鲁线虫感染性幼虫趋化作用中的活性。
