Sehrawat Anuradha, Sultana Sarwat
Section of Chemoprevention and Nutrition Toxicology, Department of Medical Elementology and Toxicology, Faculty of Science, Hamdard University, New Delhi 110 062, India.
Asian Pac J Cancer Prev. 2006 Jan-Mar;7(1):140-8.
In this communication, we document chemopreventive effects of Butea monosperma extract on hepatic carcinogenesis and on tumor promoter induced markers and oxidative stress in male Wistar rats. Treatment of male Wistar rats for five consecutive days with 2-AAF i.p. induced significant hepatic toxicity, oxidative stress and hyperproliferation. Pretreatment of B.monosperma extract (100 and 200 mg/kg body weight) prevented oxidative stress by restoring the levels of antioxidant enzymes and also prevented toxicity at both doses. The promotion parameters induced (ornithine decarboxylase activity and DNA synthesis) by 2-AAF administration in diet with partial hepatectomy (PH) were also significantly suppressed dose dependently by B. monosperma. Thereafter, we proceeded with studies on rat liver carcinogenesis. After fourteen days of DEN treatment, dietary administration of 2-AAF with PH resulted in a 100% incidence of tumors in the animals. However, B.monosperma caused reduction in the number of tumors/ rat and percentage of tumor bearing rats at the end of the study, as confirmed histologically. Thus, our data suggest that B.monosperma extract is a potent chemopreventive agent which suppresses 2-AAF-induced hepatic carcinogenesis and oxidative damage in Wistar rats. The protective activity of the plant might be due to the two major constituents (butrin and isobutrin).
在本通讯中，我们记录了紫铆提取物对雄性Wistar大鼠肝癌发生、肿瘤启动子诱导标志物及氧化应激的化学预防作用。用2-乙酰氨基芴腹腔注射连续处理雄性Wistar大鼠5天，可诱导显著的肝毒性、氧化应激和细胞过度增殖。紫铆提取物（100和200毫克/千克体重）预处理可通过恢复抗氧化酶水平预防氧化应激，且两种剂量均能预防毒性。在部分肝切除（PH）的情况下，2-乙酰氨基芴饮食给药诱导的促进参数（鸟氨酸脱羧酶活性和DNA合成）也被紫铆剂量依赖性地显著抑制。此后，我们进行了大鼠肝癌发生的研究。用二乙基亚硝胺处理14天后，在PH情况下饮食给予2-乙酰氨基芴导致动物肿瘤发生率达100%。然而，组织学证实，在研究结束时，紫铆可使每只大鼠的肿瘤数量和荷瘤大鼠百分比降低。因此，我们的数据表明，紫铆提取物是一种有效的化学预防剂，可抑制Wistar大鼠中2-乙酰氨基芴诱导的肝癌发生和氧化损伤。该植物的保护活性可能归因于两种主要成分（紫铆因和异紫铆因）。
