Suárez M, Briones H, Alarcón G, Aliaga P, Grunberg A, de Diego S, del Solar E
Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad de Chile, Santiago.
Bol Oficina Sanit Panam. 1991 Oct;111(4):319-23.
Virus isolation tests were performed on a group of 163 students who received prenatal care at the Student Medical and Dental Service at the University of Chile (Santiago) during the 1st, 2nd, and 3rd trimesters of pregnancy to determine the possible existence of genital herpes infection (isolation from vaginal secretions) or cytomegalovirus infection (isolation from urine). The samples were inoculated on cell cultures and were then studied using immunofluorescence with monoclonal antibodies. The herpes simplex virus (HSV) was detected in the vaginal secretions of 5 pregnant women, only one of whom had herpes lesions; in all these cases, the virus isolated was HSV type 2. Cytomegalovirus was detected in 7 of the pregnant women.
对一组163名学生进行了病毒分离检测。这些学生在智利大学(圣地亚哥)学生医疗和牙科服务中心接受孕期保健,孕期分别处于第一、第二和第三孕期,目的是确定是否可能存在生殖器疱疹感染(从阴道分泌物中分离)或巨细胞病毒感染(从尿液中分离)。样本接种于细胞培养物上,然后使用单克隆抗体通过免疫荧光法进行研究。在5名孕妇的阴道分泌物中检测到单纯疱疹病毒(HSV),其中只有1名孕妇有疱疹病变;在所有这些病例中,分离出的病毒均为2型HSV。在7名孕妇中检测到巨细胞病毒。