Goff Will L, Storset Anne K, Johnson Wendell C, Brown Wendy C
Animal Disease Research Unit, USDA-ARS, Pullman, Washington, USA.
Parasite Immunol. 2006 May;28(5):221-8. doi: 10.1111/j.1365-3024.2006.00830.x.
The spleen is a critical effector organ functioning, in haemoparasitic diseases like babesiosis, to destroy the pathogen and clear the host of infected erythrocytes. It has an important role in both innate responses and adaptive immune responses. Young calves demonstrate a strong spleen-dependent innate response to an initial infection with Babesia bovis involving the type-1 regulating cytokines IL-12 and IFN-gamma. However, the specific splenic cell types that produce IFN-gamma in response to infection and the cellular factors that regulate the induction have not been fully determined. Splenic NKp46(+) NK cells were identified and purified. They consisted of CD3(-), CD2(+/-), and CD8(+/-) populations. NK cells responded to exogenous IL-12 and IL-18 with the production of IFN-gamma. Functionally, IL-18 served as a potent co-stimulant with IL-12 for IFN-gamma production. Finally, innate IFN-gamma production was induced in splenic NK cells in the presence of supernatants from B. bovis merozoite-exposed monocytes in an IL-12 pathway-dependent manner.
脾脏是一个关键的效应器官，在巴贝斯虫病等血液寄生虫病中发挥作用，以破坏病原体并清除宿主体内被感染的红细胞。它在先天免疫反应和适应性免疫反应中都起着重要作用。幼牛对牛巴贝斯虫的初次感染表现出强烈的依赖脾脏的先天免疫反应，涉及1型调节细胞因子白细胞介素-12（IL-12）和干扰素-γ（IFN-γ）。然而，感染后产生IFN-γ的特定脾脏细胞类型以及调节诱导的细胞因子尚未完全确定。脾脏NKp46(+) NK细胞被识别并纯化。它们由CD3(-)、CD2(+/-)和CD8(+/-)群体组成。NK细胞对外源IL-12和IL-18有反应，并产生IFN-γ。在功能上，IL-18作为IL-12产生IFN-γ的有效共刺激剂。最后，在暴露于牛巴贝斯虫裂殖子的单核细胞的上清液存在下，脾脏NK细胞以依赖IL-12途径的方式诱导产生先天IFN-γ。
