Animal Disease Research Unit, USDA-ARS, Washington State University, Pullman, Washington 99164-6630, USA.
Parasite Immunol. 2011 Jan;33(1):34-44. doi: 10.1111/j.1365-3024.2010.01249.x.
The spleen is a critical organ in defence against haemoparasitic diseases like babesiosis. Many in vitro and ex vivo studies have identified splenic cells working in concert to activate mechanisms required for successful resolution of infection. The techniques used in those studies, however, remove cells from the anatomical context in which cell interaction and trafficking take place. In this study, an immunohistological approach was used to monitor the splenic distribution of defined cells during the acute response of naïve calves to Babesia bovis infection. Splenomegaly was characterized by disproportionate hyperplasia of large versus small leucocytes and altered distribution of several cell types thought to be important in mounting an effective immune response. In particular, the results suggest that the initial crosstalk between NK cells and immature dendritic cells occurs within the marginal zone and that immature dendritic cells are first redirected to encounter pathogens as they enter the spleen and then mature as they process antigen and migrate to T-cell-rich areas. The results of this study are remarkably similar to those observed in a mouse model of malarial infection, suggesting these dynamic events may be central to the acute response of naïve animals to haemoparasitic infection.
脾脏是抵抗血液寄生虫病（如巴贝斯虫病）的关键器官。许多体外和离体研究已经确定了协同作用的脾脏细胞激活机制，这些机制对于成功解决感染是必需的。然而，这些研究中使用的技术将细胞从细胞相互作用和运输发生的解剖学背景中去除。在这项研究中，采用免疫组织化学方法监测了新生牛对牛巴贝斯虫感染急性反应期间脾脏中特定细胞的分布。脾肿大的特点是大白细胞相对于小白细胞的不成比例增生和几种被认为对有效免疫反应很重要的细胞类型的分布改变。特别是，结果表明 NK 细胞和未成熟树突状细胞之间的初始串扰发生在边缘区，并且未成熟树突状细胞首先被重新定向以在病原体进入脾脏时遇到它们，然后在处理抗原和迁移到富含 T 细胞的区域时成熟。该研究的结果与在疟疾感染的小鼠模型中观察到的结果非常相似，这表明这些动态事件可能是新生动物对血液寄生虫感染急性反应的核心。
