Duponnois Robin, Assikbetse Komi, Ramanankierana Heriniaina, Kisa Marija, Thioulouse Jean, Lepage Michel
Institut de Recherche pour le Développement, Laboratoire des Symbioses Tropicales et Méditerranéennes, Montpellier, France.
FEMS Microbiol Ecol. 2006 May;56(2):292-303. doi: 10.1111/j.1574-6941.2006.00089.x.
The hypothesis of the present study was that the termite mounds of Macrotermes subhyalinus (MS) (a litter-forager termite) were inhabited by a specific microflora that could enhance with the ectomycorrhizal fungal development. We tested the effect of this feeding group mound material on (i) the ectomycorrhization symbiosis between Acacia holosericea (an Australian Acacia introduced in the sahelian areas) and two ectomycorrhizal fungal isolates of Scleroderma dictyosporum (IR408 and IR412) in greenhouse conditions, (ii) the functional diversity of soil microflora and (iii) the diversity of fluorescent pseudomonads. The results showed that the termite mound amendment significantly increased the ectomycorrhizal expansion. MS mound amendment and ectomycorrhizal inoculation induced strong modifications of the soil functional microbial diversity by promoting the multiplication of carboxylic acid catabolizing microorganisms. The phylogenetic analysis showed that fluorescent pseudomonads mostly belong to the Pseudomonads monteillii species. One of these, P. monteillii isolate KR9, increased the ectomycorrhizal development between S. dictyosporum IR412 and A. holosericea. The occurrence of MS termite mounds could be involved in the expansion of ectomycorrhizal symbiosis and could be implicated in nutrient flow and local diversity.
本研究的假设是，亚透明白蚁（Macrotermes subhyalinus，MS）（一种采食枯枝落叶的白蚁）的蚁丘中栖息着特定的微生物群落，该群落能够随着外生菌根真菌的发育而增加。我们测试了这种取食群体的蚁丘材料对以下方面的影响：（i）在温室条件下，全缘相思树（Acacia holosericea，一种引入萨赫勒地区的澳大利亚金合欢）与硬皮马勃菌（Scleroderma dictyosporum）的两种外生菌根真菌分离株（IR408和IR412）之间的外生菌根共生关系；（ii）土壤微生物群落的功能多样性；（iii）荧光假单胞菌的多样性。结果表明，白蚁蚁丘改良剂显著增加了外生菌根的扩展。MS蚁丘改良剂和外生菌根接种通过促进羧酸分解微生物的繁殖，引起了土壤功能微生物多样性的强烈变化。系统发育分析表明，荧光假单胞菌大多属于蒙氏假单胞菌（Pseudomonads monteillii）物种。其中一种蒙氏假单胞菌分离株KR9，增加了硬皮马勃菌IR412与全缘相思树之间的外生菌根发育。MS白蚁蚁丘的存在可能参与了外生菌根共生的扩展，并可能与养分流动和局部多样性有关。
