Aguirre E, Tesouro M A, Ruiz L, Amusategui I, Sainz A
Departamento de Medicina y Cirugía Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain.
J Vet Med B Infect Dis Vet Public Health. 2006 May;53(4):197-200. doi: 10.1111/j.1439-0450.2006.00937.x.
This paper reports the first genetic characterization of Anaplasma (Ehrlichia) platys in Spain from a naturally infected dog. The dog presented clinical signs compatible with canine ehrlichiosis. After DNA extraction and PCR amplification, 16S rRNA gene and citrate synthase gene ( gltA) of this agent were amplified. The GenBank accession number for the nucleotide sequence of the 16S rRNA gene of this strain is AY530806. The A. platys strains registered in France and Japan showed the highest similarity to the 16S rRNA gene sequence obtained from the Spanish strain. In the amplification of the gltA gene, a 1443 bp fragment was obtained, and three nucleotide differences were detected in comparison with other strains sequences. These data confirm the presence of A. platys in a dog showing clinical signs compatible with ehrlichiosis in Spain.
本文报道了西班牙首例来自自然感染犬的扁平无形体（埃立克体）的基因特征。该犬呈现出与犬埃立克体病相符的临床症状。经DNA提取和PCR扩增后，扩增出了该病原体的16S rRNA基因和柠檬酸合酶基因（gltA）。该菌株16S rRNA基因核苷酸序列的GenBank登录号为AY530806。在法国和日本登记的扁平无形体菌株与从西班牙菌株获得的16S rRNA基因序列显示出最高的相似性。在gltA基因扩增中，获得了一个1443 bp的片段，与其他菌株序列相比检测到三个核苷酸差异。这些数据证实了在西班牙一只呈现出与埃立克体病相符临床症状的犬中存在扁平无形体。
