Institute for Infectious Diseases and Zoonoses, Department for Veterinary Sciences, Faculty for Veterinary Medicine, LMU Munich, Veterinärstrasse 13, 80539 Munich, Germany.
Parasit Vectors. 2012 Mar 9;5:49. doi: 10.1186/1756-3305-5-49.
It is known that Anaplasma (A.) platys, the causative agent of infectious canine cyclic thrombocytopenia, is endemic in countries of the Mediterranean basin. However, few reports are available from the Balkans. This case report describes a dog, which was imported from Croatia to Germany in May 2010. One month later the dog was presented to a local veterinarian in Germany due to intermittent/recurrent diarrhoea. Diagnostic tests were performed to identify infections caused by Anaplasma spp., Ehrlichia spp., Hepatozoon canis, Babesia spp., Leishmania spp., Borrelia burgdorferi and/or Dirofilaria immitis.
Haematological examination of a blood smear revealed basophilic inclusions in thrombocytes, which were confirmed as A. platys with a species-specific real-time PCR. Additionally, an infection with Babesia (B.) vogeli was also detected (PCR and serology). No specific antibodies against Anaplasma antigen were detectable. Although the dog showed no specific clinical signs, thrombocytopenia, anaemia and elevated C-reactive protein (CRP) were observed. Sequencing of a 1,348-bp partial ribosomal RNA gene revealed highest homology to A. platys sequences from Thailand, Japan and France.
A. platys was detected for first time in a dog imported from Croatia. As the dog was also co-infected by B. vogeli, unique serological and haematological findings were recorded. Thrombocytopenia, anaemia and elevated values of C-reactive protein were the laboratory test abnormalities observed in this case. A. platys infections should be considered in dogs coming from Croatia and adjacent regions.
已知感染犬传染性循环血小板减少症的病原体安氏（A.）platys 在地中海盆地国家流行。然而，来自巴尔干半岛的报道很少。本病例报告描述了一只于 2010 年 5 月从克罗地亚进口到德国的狗。一个月后，这只狗因间歇性/复发性腹泻到德国当地兽医处就诊。进行了诊断测试以确定由安氏（Anaplasma） spp.、埃立克体（Ehrlichia） spp.、犬肝孢子虫（Hepatozoon canis）、巴贝斯虫（Babesia） spp.、利什曼原虫（Leishmania） spp.、伯氏疏螺旋体（Borrelia burgdorferi）和/或犬恶丝虫（Dirofilaria immitis）引起的感染。
血液涂片的血液学检查显示血小板中有嗜碱性包涵体，用种特异性实时 PCR 证实为 A. platys。此外，还检测到感染了巴贝斯虫（B.） vogeli（PCR 和血清学）。未检测到针对安氏抗原的特异性抗体。尽管该犬没有表现出特定的临床症状，但观察到血小板减少症、贫血和 C 反应蛋白（CRP）升高。1,348bp 部分核糖体 RNA 基因的测序显示与来自泰国、日本和法国的 A. platys 序列具有最高同源性。
首次在一只从克罗地亚进口的狗中检测到 A. platys。由于该犬还同时感染了 B. vogeli，因此记录了独特的血清学和血液学发现。在这种情况下，观察到的实验室测试异常包括血小板减少症、贫血和 C 反应蛋白升高。来自克罗地亚和邻近地区的狗应考虑感染 A. platys。
