通过¹H-和¹³C-核磁共振光谱法对大麦β-D-葡聚糖酶促降解产生的寡糖进行结构归属
Assignment of structures to oligosaccharides produced by enzymic degradation of a beta-D-glucan from barley by 1H- and 13C-n.m.r. spectroscopy.
作者信息
Bock K, Duus J O, Norman B, Pedersen S
机构信息
Department of Chemistry, Carlsberg Laboratory, Gl. Carlsberg Vej 10. Valby, Denmark.
出版信息
Carbohydr Res. 1991 Apr 24;211(2):219-33. doi: 10.1016/0008-6215(91)80093-3.
The structures of one tri-(1), two tetra-(2 and 3), and one hexa-saccharide (4) produced by treatment of barley flour, after removal of the starch components, with a fungal beta-D-glucanase (Finizyme) have been assigned on the basis of 1H- and 13C-n.m.r. data as follows: beta-D-Glcp-(1----3)-beta-D-Glcp-(1----4)-D-Glcp (1), beta-D-Glcp-(1----4)-beta-D-Glcp-(1----3)-beta-D-Glcp-(1----4)-D-Glcp (2), beta-D-Glcp-(1----3)-beta-D-Glcp-(1----4)-beta-D-Glcp-(1----4)-D-Glcp (3), and beta-D-Xylp-(1----4)-[alpha-L-Araf-(1----3)]-[alpha-L-Ara f-(1----2)-beta-D-Xylp-(1----4)-beta-D-Xylp- (1----4)-D-Xylp (4).
在用真菌β-D-葡聚糖酶（Finizyme）处理去除淀粉成分后的大麦粉所产生的一种三糖（1）、两种四糖（2和3）和一种六糖（4）的结构，已根据1H和13C核磁共振数据确定如下：β-D-葡萄糖吡喃糖基-(1→3)-β-D-葡萄糖吡喃糖基-(1→4)-D-葡萄糖（1），β-D-葡萄糖吡喃糖基-(1→4)-β-D-葡萄糖吡喃糖基-(1→3)-β-D-葡萄糖吡喃糖基-(1→4)-D-葡萄糖（2），β-D-葡萄糖吡喃糖基-(1→3)-β-D-葡萄糖吡喃糖基-(1→4)-β-D-葡萄糖吡喃糖基-(1→4)-D-葡萄糖（3），以及β-D-木糖吡喃糖基-(1→4)-[α-L-阿拉伯呋喃糖基-(1→3)]-[α-L-阿拉伯呋喃糖基-(1→2)-β-D-木糖吡喃糖基-(1→4)-β-D-木糖吡喃糖基-(1→4)-D-木糖（4）。
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