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地衣芽孢杆菌内切-1,3-1,4-β-D-葡聚糖4-葡聚糖水解酶酶促作用产物的立体化学过程和结构

Stereochemical course and structure of the products of the enzymic action of endo-1,3-1,4-beta-D-glucan 4-glucanohydrolase from Bacillus licheniformis.

作者信息

Malet C, Jiménez-Barbero J, Bernabé M, Brosa C, Planas A

机构信息

Department de Química Orgànica, CETS Institut Químic de Sarrià, Universitat Ramón Llull, Barcelona, Spain.

出版信息

Biochem J. 1993 Dec 15;296 ( Pt 3)(Pt 3):753-8. doi: 10.1042/bj2960753.

DOI:10.1042/bj2960753
PMID:8280073
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1137759/
Abstract

The stereochemical course of the reaction catalysed by endo-1,3-1,4-beta-D-glucan 4-glucanohydrolase (EC 3.2.1.73) has been determined by 1H n.m.r. The enzyme-catalysed hydrolysis of barley beta-glucan proceeds with overall retention of the anomeric configuration, indicating that the enzyme operates through a double-displacement mechanism. The structures of the final oligosaccharide products, 3-beta-O-cellobiosyl D-glucopyranoside and 3-beta-O-cellotriosyl D-glucopyranoside, have been completely assigned by 1H- and 13C-n.m.r. spectroscopy.

摘要

通过核磁共振氢谱（1H n.m.r.）确定了内切-1,3-1,4-β-D-葡聚糖4-葡聚糖水解酶（EC 3.2.1.73）催化反应的立体化学过程。大麦β-葡聚糖的酶促水解反应在总体上保留了异头构型，这表明该酶通过双取代机制起作用。最终寡糖产物3-β-O-纤维二糖基-D-吡喃葡萄糖苷和3-β-O-纤维三糖基-D-吡喃葡萄糖苷的结构已通过核磁共振氢谱（1H-n.m.r.）和核磁共振碳谱（13C-n.m.r.）光谱完全确定。

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Stereochemical course and structure of the products of the enzymic action of endo-1,3-1,4-beta-D-glucan 4-glucanohydrolase from Bacillus licheniformis.地衣芽孢杆菌内切-1,3-1,4-β-D-葡聚糖4-葡聚糖水解酶酶促作用产物的立体化学过程和结构
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