地衣芽孢杆菌内切-1,3-1,4-β-D-葡聚糖4-葡聚糖水解酶酶促作用产物的立体化学过程和结构
Stereochemical course and structure of the products of the enzymic action of endo-1,3-1,4-beta-D-glucan 4-glucanohydrolase from Bacillus licheniformis.
作者信息
Malet C, Jiménez-Barbero J, Bernabé M, Brosa C, Planas A
机构信息
Department de Química Orgànica, CETS Institut Químic de Sarrià, Universitat Ramón Llull, Barcelona, Spain.
出版信息
Biochem J. 1993 Dec 15;296 ( Pt 3)(Pt 3):753-8. doi: 10.1042/bj2960753.
Abstract
The stereochemical course of the reaction catalysed by endo-1,3-1,4-beta-D-glucan 4-glucanohydrolase (EC 3.2.1.73) has been determined by 1H n.m.r. The enzyme-catalysed hydrolysis of barley beta-glucan proceeds with overall retention of the anomeric configuration, indicating that the enzyme operates through a double-displacement mechanism. The structures of the final oligosaccharide products, 3-beta-O-cellobiosyl D-glucopyranoside and 3-beta-O-cellotriosyl D-glucopyranoside, have been completely assigned by 1H- and 13C-n.m.r. spectroscopy.
摘要
通过核磁共振氢谱（1H n.m.r.）确定了内切-1,3-1,4-β-D-葡聚糖4-葡聚糖水解酶（EC 3.2.1.73）催化反应的立体化学过程。大麦β-葡聚糖的酶促水解反应在总体上保留了异头构型，这表明该酶通过双取代机制起作用。最终寡糖产物3-β-O-纤维二糖基-D-吡喃葡萄糖苷和3-β-O-纤维三糖基-D-吡喃葡萄糖苷的结构已通过核磁共振氢谱（1H-n.m.r.）和核磁共振碳谱（13C-n.m.r.）光谱完全确定。
相似文献
1
Stereochemical course and structure of the products of the enzymic action of endo-1,3-1,4-beta-D-glucan 4-glucanohydrolase from Bacillus licheniformis.地衣芽孢杆菌内切-1,3-1,4-β-D-葡聚糖4-葡聚糖水解酶酶促作用产物的立体化学过程和结构
Biochem J. 1993 Dec 15;296 ( Pt 3)(Pt 3):753-8. doi: 10.1042/bj2960753.
2
Stereochemical course of glucan hydrolysis by barley (1-->3)- and (1-->3, 1-->4)-beta-glucanases.大麦（1→3）-和（1→3，1→4）-β-葡聚糖酶水解葡聚糖的立体化学过程。
Biochim Biophys Acta. 1995 Nov 15;1253(1):112-6. doi: 10.1016/0167-4838(95)00157-p.
3
Direct 1H n.m.r. determination of the stereochemical course of hydrolyses catalysed by glucanase components of the cellulase complex.通过纤维素酶复合物的葡聚糖酶组分催化的水解反应立体化学过程的直接¹H核磁共振测定。
Biochem Biophys Res Commun. 1986 Sep 14;139(2):487-94. doi: 10.1016/s0006-291x(86)80017-1.
4
Mechanism of Bacillus 1,3-1,4-beta-D-glucan 4-glucanohydrolases: kinetics and pH studies with 4-methylumbelliferyl beta-D-glucan oligosaccharides.芽孢杆菌1,3-1,4-β-D-葡聚糖4-葡聚糖水解酶的作用机制：对4-甲基伞形酮基-β-D-葡聚糖寡糖的动力学及pH研究
Biochemistry. 1997 Nov 11;36(45):13838-48. doi: 10.1021/bi9711341.
5
Synthesis of aryl 3-O-beta-cellobiosyl-beta-D-glucopyranosides for reactivity studies of 1,3-1,4-beta-glucanases.用于1,3-1,4-β-葡聚糖酶反应性研究的芳基3-O-β-纤维二糖基-β-D-吡喃葡萄糖苷的合成。
Carbohydr Res. 1998 Aug;310(1-2):53-64. doi: 10.1016/s0008-6215(98)00175-x.
6
Structural basis for the substrate specificity of a Bacillus 1,3-1,4-beta-glucanase.芽孢杆菌1,3-1,4-β-葡聚糖酶底物特异性的结构基础
J Mol Biol. 2006 Apr 7;357(4):1211-25. doi: 10.1016/j.jmb.2006.01.014. Epub 2006 Jan 25.
7
8
Enzymically produced cyclic alpha-1,3-linked and alpha-1,6-linked oligosaccharides of D-glucose.通过酶促反应生成的D-葡萄糖的环状α-1,3-连接和α-1,6-连接的低聚糖。
Eur J Biochem. 1994 Dec 1;226(2):641-8. doi: 10.1111/j.1432-1033.1994.tb20091.x.
9
A specific chromophoric substrate for activity assays of 1,3-1,4-beta-D-glucan 4-glucanohydrolases.一种用于1,3-1,4-β-D-葡聚糖4-葡聚糖水解酶活性测定的特异性发色底物。
J Biotechnol. 1996 Jul 31;48(3):209-19. doi: 10.1016/0168-1656(96)01511-8.
10
Expeditious synthesis of a new hexasaccharide using transglycosylation reaction catalyzed by Bacillus (1-->3),(1-->4)-Beta-D-glucan 4-glucanohydrolase.利用芽孢杆菌（1→3），（1→4）-β-D-葡聚糖4-葡聚糖水解酶催化的转糖基化反应快速合成一种新的六糖。
Carbohydr Res. 1998 Sep;311(1-2):95-9. doi: 10.1016/s0008-6215(98)00212-2.
引用本文的文献
1
Molecular insights into the hydrolysis and transglycosylation of a deep-sea -derived GH16 family laminarinase.深海来源 GH16 家族昆布多糖酶的水解和转糖苷作用的分子见解。
Appl Environ Microbiol. 2024 Oct 23;90(10):e0094224. doi: 10.1128/aem.00942-24. Epub 2024 Sep 17.
2
Crystallographic insight into the evolutionary origins of xyloglucan endotransglycosylases and endohydrolases.对木葡聚糖内转糖基酶和内切水解酶进化起源的晶体学见解。
Plant J. 2017 Feb;89(4):651-670. doi: 10.1111/tpj.13421. Epub 2017 Feb 11.
3
Pre-steady-state kinetics of Bacillus licheniformis 1,3-1,4-beta-glucanase: evidence for a regulatory binding site.地衣芽孢杆菌1,3-1,4-β-葡聚糖酶的前稳态动力学：存在调节性结合位点的证据
Biochem J. 2003 May 1;371(Pt 3):997-1003. doi: 10.1042/BJ20021504.
4
Presteady-state kinetics of Bacillus 1,3-1,4-beta-glucanase: binding and hydrolysis of a 4-methylumbelliferyl trisaccharide substrate.芽孢杆菌1,3-1,4-β-葡聚糖酶的前稳态动力学：4-甲基伞形酮三糖底物的结合与水解
Biochem J. 2001 Jul 1;357(Pt 1):195-202. doi: 10.1042/0264-6021:3570195.
本文引用的文献
1
Notes on sugar determination.糖分测定笔记。
J Biol Chem. 1952 Mar;195(1):19-23.
2
Enzymatic properties of a beta-glucanase from Bacillus subtilis.枯草芽孢杆菌β-葡聚糖酶的酶学性质
J Biol Chem. 1961 Nov;236:2858-62.
3
Molecular and active-site structure of a Bacillus 1,3-1,4-beta-glucanase.一种芽孢杆菌1,3-1,4-β-葡聚糖酶的分子结构与活性位点结构
Proc Natl Acad Sci U S A. 1993 Jun 1;90(11):5287-91. doi: 10.1073/pnas.90.11.5287.
4
Characterization, cloning and sequencing of a thermostable endo-(1,3-1,4) beta-glucanase-encoding gene from an alkalophilic Bacillus brevis.嗜碱短芽孢杆菌中一个编码耐热性内切（1,3 - 1,4）β - 葡聚糖酶基因的鉴定、克隆及测序
Appl Microbiol Biotechnol. 1993 Jan;38(4):507-13. doi: 10.1007/BF00242946.
5
Molecular cloning and expression of a Bacillus subtilis beta-glucanase gene in Escherichia coli.枯草芽孢杆菌β-葡聚糖酶基因在大肠杆菌中的分子克隆与表达
Gene. 1983 Aug;23(2):211-9. doi: 10.1016/0378-1119(83)90053-7.
6
Mutation of active site residues in synthetic T4-lysozyme gene and their effect on lytic activity.合成T4溶菌酶基因活性位点残基的突变及其对裂解活性的影响。
Biochem Biophys Res Commun. 1988 Jun 16;153(2):862-8. doi: 10.1016/s0006-291x(88)81175-6.
7
Site-directed mutagenesis of beta-galactosidase (E. coli) reveals that tyr-503 is essential for activity.β-半乳糖苷酶（大肠杆菌）的定点诱变表明，酪氨酸-503对其活性至关重要。
Biochem Biophys Res Commun. 1988 May 16;152(3):1050-5. doi: 10.1016/s0006-291x(88)80390-5.
8
The beta-glucanase gene from Bacillus amyloliquefaciens shows extensive homology with that of Bacillus subtilis.解淀粉芽孢杆菌的β-葡聚糖酶基因与枯草芽孢杆菌的β-葡聚糖酶基因具有广泛的同源性。
Gene. 1986;49(2):177-87. doi: 10.1016/0378-1119(86)90278-7.
9
Direct 1H n.m.r. determination of the stereochemical course of hydrolyses catalysed by glucanase components of the cellulase complex.通过纤维素酶复合物的葡聚糖酶组分催化的水解反应立体化学过程的直接¹H核磁共振测定。
Biochem Biophys Res Commun. 1986 Sep 14;139(2):487-94. doi: 10.1016/s0006-291x(86)80017-1.
10
Biotechnology aids to improve feed and feed digestion: enzymes and fermentation.生物技术有助于改善饲料及饲料消化：酶与发酵。
Arch Tierernahr. 1989 Mar;39(3):241-61. doi: 10.1080/17450398909429530.
Zotero 插件