Kim Seong-Jin, Lee Su-A, Yun Sook Jung, Kim Jong-Keun, Park Jong-Seong, Jeong Han-Seong, Lee Jeung-Hoon, Moon Seong-Joon, Won Young-Ho
Department of Dermatology, Chonnam National University, Gwangju, South Korea.
Exp Dermatol. 2006 May;15(5):362-7. doi: 10.1111/j.0906-6705.2006.00418.x.
Although the vanilloid receptor 1 (VR1) was originally discovered on primary sensory neurons, its broad tissue expression in non-neuronal cells has been reported on. Recently, VR1 expression was clearly demonstrated in a variety of cutaneous components, such as keratinocytes, glandular epithelium, mast cells and sebocytes, except for melanocytes and fibroblasts. However, we demonstrated the VR1 expression in the cultured human skin fibroblasts as follows. Previously cloned human VR1 primers that corresponded to the expected size of 680 bp by reverse transcriptase polymerase chain reaction were identified on the fibroblasts, the same as was noted for the positive control, the HaCaT cells. A positive immunoreactivity of the VR1 was observed both on fibroblasts and on HaCaT cells by Western blotting analysis. Fibroblasts treated with capsaicin, an agonist to the VR1, induced significant changes of the membrane current and the intracellular calcium level, and these changes were antagonized by capsazepin. Capsaicin treatment also showed a positive immunocytochemistry result. Our results suggest the existence of VR1 on fibroblasts; this receptor is likely to be influenced by ligand-dependent activation.
尽管香草酸受体1（VR1）最初是在初级感觉神经元上发现的，但已有报道称其在非神经元细胞中广泛表达。最近，除黑素细胞和成纤维细胞外，VR1在多种皮肤成分中均有明显表达，如角质形成细胞、腺上皮、肥大细胞和皮脂腺细胞。然而，我们如下证明了培养的人皮肤成纤维细胞中VR1的表达。通过逆转录聚合酶链反应，在成纤维细胞上鉴定出先前克隆的与预期大小680 bp相对应的人VR1引物，与阳性对照HaCaT细胞的情况相同。通过蛋白质免疫印迹分析，在成纤维细胞和HaCaT细胞上均观察到VR1的阳性免疫反应性。用VR1激动剂辣椒素处理的成纤维细胞，可诱导膜电流和细胞内钙水平发生显著变化，而这些变化可被辣椒平拮抗。辣椒素处理也显示出阳性免疫细胞化学结果。我们的结果表明成纤维细胞上存在VR1；该受体可能受配体依赖性激活的影响。
