Barile S, Medda E, Nisticò L, Bordignon V, Cordiali-Fei P, Carducci M, Rainaldi A, Marinelli R, Bonifati C
Department of Dermatology, S. Gallicano Institute, Rome, Italy.
Exp Dermatol. 2006 May;15(5):368-76. doi: 10.1111/j.0906-6705.2006.00416.x.
We investigated the relationship between eight polymorphisms in the gene encoding for vascular endothelial growth factor (VEGF) (-1540C > A, -1512Ins18, -1451C > T, -460T > C, -160C > T, -152G > A, -116G > A and +405G > C) and plaque-type psoriasis stratified for age at onset, gender and family history of dermatosis. For this purpose, 117 patients with chronic plaque-type psoriasis and 215 healthy subjects were enrolled. We found that being homozygous -1540AA, -1512InsIns, -1451TT, -460CC and -152AA conferred a significant risk in developing psoriasis compared with heterozygous (-1540CA, -1512 + Ins, -1451CT, -460CT and -152AG) and homozygous genotypes (-1540CC, -1512 + +-1451CC, -460TT and -152GG) grouped together [odds ratio (ORs) = 1.73, 1.73, 1.73, 1.77 and 1.87, respectively]. Conversely, having the -116AA or +405GG genotype did not significantly increase the risk of disease expression compared with other genotypes of the same loci. Interestingly, we found that -1540AA, -1512InsIns, -1451TT, -460CC and -152AA homozygous genotypes have a significant two-fold increased risk in developing psoriasis after the age of 40 years (late-onset psoriasis) (ORs = 2.19, 2.19, 2.19, 2.05 and 2.26; P = 0.02, 0.02, 0.02, 0.04 and 0.02, respectively) as compared with controls. On the contrary, we found no phenotype-genotype association of the same magnitude among the patients in whom psoriasis developed at or before the age of 40 years (early-onset psoriasis) compared with controls. Genotype distributions were not significantly different when cases and controls were stratified either by gender or family history of psoriasis. Finally, VEGF plasma concentration was not significantly different between patients and controls and was not correlated with the severity of the disease.
我们研究了血管内皮生长因子（VEGF）编码基因中的8种多态性（-1540C＞A、-1512Ins18、-1451C＞T、-460T＞C、-160C＞T、-152G＞A、-116G＞A和+405G＞C）与按发病年龄、性别和皮肤病家族史分层的斑块型银屑病之间的关系。为此，招募了117例慢性斑块型银屑病患者和215名健康受试者。我们发现，与杂合子（-1540CA、-1512 + Ins、-1451CT、-460CT和-152AG）以及合并在一起的纯合子基因型（-1540CC、-1512 + +、-1451CC、-460TT和-152GG）相比，-1540AA、-1512InsIns、-1451TT、-460CC和-152AA纯合子在患银屑病方面具有显著更高的风险[比值比（ORs）分别为1.73、1.73、1.73、1.77和1.87]。相反，与同一基因座的其他基因型相比，-116AA或+405GG基因型并未显著增加疾病表达风险。有趣的是，我们发现-1540AA、-1512InsIns、-1451TT、-460CC和-152AA纯合子基因型在40岁之后患银屑病（迟发性银屑病）的风险显著增加两倍（ORs分别为2.19、2.19、2.19、2.05和2.26；P值分别为0.02、0.02、0.02、0.04和0.02）。相反，我们发现在40岁及之前发病的银屑病患者（早发性银屑病）与对照组之间，未观察到相同程度的表型-基因型关联。当根据性别或银屑病家族史对病例和对照进行分层时，基因型分布没有显著差异。最后，患者与对照组之间的VEGF血浆浓度没有显著差异，且与疾病严重程度无关。
