Gorzelanny Christian, Goerge Tobias, Schnaeker Eva-Maria, Thomas Kai, Luger Thomas A, Schneider Stefan W
Department of Dermatology, University of Muenster, Muenster, Germany.
Exp Dermatol. 2006 May;15(5):387-91. doi: 10.1111/j.0906-6705.2006.00424.x.
This study demonstrates an innovative application of atomic force microscopy (AFM). The combination of high-resolution AFM technology and tape stripping is presented as a tool for the structure analysis of human stratum corneum (SC) at a nanometer scale. Topographic images with a vertical resolution of about 10 nm of the SC are presented. Topographical and structural differences between aged and young skin can be observed. Aged skin SC is characterized by an increased single-cell surface area, prominent intercellular gaps and enhanced cell surface roughness. The use of AFM in combination with other already established methods, e.g. tape stripping in the field of dermatological research will give new insights to the structure, function and morphodynamics of SC.
本研究展示了原子力显微镜（AFM）的一种创新应用。高分辨率AFM技术与胶带剥离相结合，作为一种在纳米尺度上对人体角质层（SC）进行结构分析的工具被提出。展示了垂直分辨率约为10 nm的SC的形貌图像。可以观察到老年皮肤和年轻皮肤之间的形貌和结构差异。老年皮肤SC的特征是单细胞表面积增加、细胞间间隙突出以及细胞表面粗糙度增加。在皮肤病学研究领域，将AFM与其他已确立的方法（如胶带剥离）结合使用，将为SC的结构、功能和形态动力学提供新的见解。
