Aoe Keisuke, Hiraki Akio, Murakami Tomoyuki, Toyooka Shinichi, Shivapurkar Narayan, Gazdar Adi F, Sueoka Naoko, Taguchi Koji, Kamei Toshiaki, Takeyama Hiroyasu, Sugi Kazuro, Kishimoto Takumi
Department of Respiratory Medicine, NHO Sanyo National Hospital, 685 Higashi-kiwa, Ube, Yamaguchi 755-0241, Japan.
Cancer Sci. 2006 Apr;97(4):292-5. doi: 10.1111/j.1349-7006.2006.00171.x.
Malignant mesothelioma is the most common primary pleural neoplasm. Association of simian virus 40 (SV40) with malignant mesothelioma has been reported, suggesting that SV40 plays an important role in the origin of a subset of these tumors. However, significant geographic variation is present as to how often this association occurs. As no study concerning SV40 in malignant mesothelioma has been reported from Japan, we examined the frequency of SV40 infection in Japanese malignant mesothelioma cases. In pleural malignant mesothelioma tissue from 35 patients in Japan, we sought the presence of SV40 large T antigen DNA using real-time polymerase chain reaction (PCR), as well as expression of the viral protein using immunohistological methods. Real-time PCR demonstrated that two of 35 mesotheliomas contained DNA sequences encoding portions of SV40 large T antigen. None of the 35 malignant mesothelioma specimens showed immunoreactivity for SV40 large T antigen. SV40 infection does not appear to have a major role in the development of malignant mesothelioma in Japan.
恶性间皮瘤是最常见的原发性胸膜肿瘤。有报道称猿猴病毒40（SV40）与恶性间皮瘤有关联，这表明SV40在这些肿瘤的一部分起源中起重要作用。然而，这种关联发生的频率存在显著的地理差异。由于日本尚未有关于恶性间皮瘤中SV40的研究报道，我们检测了日本恶性间皮瘤病例中SV40感染的频率。在来自日本35例患者的胸膜恶性间皮瘤组织中，我们使用实时聚合酶链反应（PCR）寻找SV40大T抗原DNA的存在，并使用免疫组织学方法检测病毒蛋白的表达。实时PCR显示，35例间皮瘤中有2例含有编码SV40大T抗原部分的DNA序列。35例恶性间皮瘤标本中没有一个对SV40大T抗原显示免疫反应性。在日本，SV40感染似乎在恶性间皮瘤的发生中不起主要作用。
