温和气单胞菌溶血素通过增加HCO3-的分泌导致腹泻。

Aeromonas sobria hemolysin causes diarrhea by increasing secretion of HCO3-.

作者信息

Takahashi Akira, Nakano Masayuki, Okamoto Keinosuke, Fujii Yoshio, Mawatari Kazuaki, Harada Nagakatsu, Nakaya Yutaka

机构信息

Department of Nutrition and Metabolism, The University of Tokushima Graduate School, Institute of Health Biosciences, Tokushima City, Tokushima, Japan.

出版信息

FEMS Microbiol Lett. 2006 May;258(1):92-5. doi: 10.1111/j.1574-6968.2006.00204.x.

DOI:10.1111/j.1574-6968.2006.00204.x

PMID:16630261

Abstract

Aeromonas sobria hemolysin (ASH) is one of the major virulence factors produced by A. sobria, a causative agent of diarrhea in humans. We investigated the effects of ASH on anion transport in human colonic epithelial cells. ASH increased short circuit currents across the intestinal epithelia, which were suppressed by anion channel antagonists, such as carbonic anhydrase inhibitors, and by the removal of external HCO3-. Iliac fluid accumulation was also inhibited by carbonic anhydrase inhibitors. The results suggest that ASH activates HCO3- secretion, whose level correlates with the severity of diarrhea.

摘要

温和气单胞菌溶血素（ASH）是由温和气单胞菌产生的主要毒力因子之一，温和气单胞菌是人类腹泻的病原体。我们研究了ASH对人结肠上皮细胞阴离子转运的影响。ASH增加了跨肠上皮的短路电流，而这种电流被阴离子通道拮抗剂（如碳酸酐酶抑制剂）以及去除细胞外HCO3-所抑制。碳酸酐酶抑制剂也抑制了肠液积聚。结果表明，ASH激活了HCO3-分泌，其水平与腹泻的严重程度相关。