Sexton Adrienne C, Cozijnsen Anton J, Keniry Andrew, Jewell Erica, Love Christopher G, Batley Jacqueline, Edwards David, Howlett Barbara J
School of Botany, The University of Melbourne, Parkville, VIC, Australia.
FEMS Microbiol Lett. 2006 May;258(1):150-60. doi: 10.1111/j.1574-6968.2006.00212.x.
The ascomycete Sclerotinia sclerotiorum is a plant pathogen with a very broad host range. In order to identify and characterize genes involved in S. sclerotiorum infection of Brassica napus (canola), expressed sequence tags (ESTs) were examined from libraries prepared from three tissues: complex appressorium (infection cushions), mycelia grown on agar and lesions formed on leaves of B. napus. A high proportion of genes (68%) had not been previously reported for S. sclerotiorum in public gene or EST databases. The types of novel genes identified in the infection cushion library highlights the functional specificity of these structures and similarities to appressoria in other fungal pathogens. Quantitative real-time PCR was used to analyse tissue specificity and timing of transcription of genes with best matches to MAS3 (appressoria-associated protein from Magnaporthe grisea), cellobiohydrolase I, oxaloacetate acetylhydrolase, metallothionein, pisatin demethylase, and an unknown gene with orthologs in fungal pathogens but not in saprophytic fungi.
子囊菌核盘菌是一种寄主范围非常广泛的植物病原体。为了鉴定和表征参与核盘菌侵染甘蓝型油菜（油菜）的基因，从由三种组织制备的文库中检测了表达序列标签（EST）：复合附着胞（侵染垫）、在琼脂上生长的菌丝体以及在甘蓝型油菜叶片上形成的病斑。在公共基因或EST数据库中，之前尚未报道过核盘菌的高比例基因（68%）。在侵染垫文库中鉴定出的新基因类型突出了这些结构的功能特异性以及与其他真菌病原体附着胞的相似性。使用定量实时PCR分析与MAS3（来自稻瘟病菌的附着胞相关蛋白）、纤维二糖水解酶I、草酰乙酸乙酰水解酶、金属硫蛋白、豌豆素脱甲基酶以及在真菌病原体中有直系同源物但在腐生真菌中没有的一个未知基因最佳匹配的基因的组织特异性和转录时间。
