Kövesi György, Szende Béla
Department of Periodontology, Semmelweis University Faculty of Dentistry, Mária, Hungary.
J Oral Pathol Med. 2006 May;35(5):274-7. doi: 10.1111/j.1600-0714.2006.00396.x.
Studies on the expression of genes regulating cell proliferation and apoptosis are essential to help better understand the severity and possible malignant transformation of oral leukoplakia.
The characteristics of cyclin D1, p27, and p63 were investigated in this microscopic study, complementing our previous results with Ki67, p53, and the apoptosis index. Clinical and histologic as well as immunohistochemical studies were carried out on oral leukoplakia of 18 patients. Homogenous, or non-homogenous (nodular or speckled) and erythroleukoplakia were determined clinically. Pathologic classification was performed according to the degree of dysplasia. Immunoperoxidase reaction for cyclin D1, p27, and p63 was carried out on the biopsy specimens and the positivity of the reactions was calculated for 1000 epithelial cells.
The expression of cyclin D1 increased in parallel with the severity of leukoplakia. The p27 index was 14-16% in homogenous and nodular leukoplakias but it was substantially lower to 1-2% in erythroleukoplakia. The p63 index was 10% in homogenous, 5% in nodular or speckled, but nearly 20% in erythroleukoplakia, on the average.
These results suggest that the characteristic expression of cyclin D1, p27, and p63 in various forms of leukoplakia may be of prognostic value.
研究调节细胞增殖和凋亡的基因表达，对于更好地理解口腔白斑的严重程度及可能的恶变至关重要。
本显微镜研究调查了细胞周期蛋白D1、p27和p63的特征，以补充我们之前关于Ki67、p53和凋亡指数的研究结果。对18例口腔白斑患者进行了临床、组织学及免疫组化研究。临床上确定为均质型、非均质型（结节状或斑点状）及红白斑。根据发育异常程度进行病理分类。对活检标本进行细胞周期蛋白D1、p27和p63的免疫过氧化物酶反应，并计算1000个上皮细胞的反应阳性率。
细胞周期蛋白D1的表达随白斑严重程度平行增加。均质型和结节状白斑的p27指数为14% - 16%，但红白斑中该指数显著降低至1% - 2%。平均而言，均质型白斑的p63指数为10%，结节状或斑点状白斑为5%，而红白斑接近20%。
这些结果表明，细胞周期蛋白D1、p27和p63在各种形式白斑中的特征性表达可能具有预后价值。
