单克隆抗小鼠层粘连蛋白抗体：AL-1与层粘连蛋白α1链反应，AL-2与层粘连蛋白β1链反应，AL-4与层粘连蛋白β1链的卷曲螺旋结构域反应。

Monoclonal anti-mouse laminin antibodies: AL-1 reacts with laminin alpha1 chain, AL-2 with laminin beta1 chain, and AL-4 with the coiled-coil domain of laminin beta1 chain.

作者信息

Schéele Susanne, Sasaki Takako, Arnal-Estapé Anna, Durbeej Madeleine, Ekblom Peter

机构信息

Department of Experimental Medical Science, BMC B12, Lund University, Lund, Sweden.

出版信息

Matrix Biol. 2006 Jul;25(5):301-5. doi: 10.1016/j.matbio.2006.03.004. Epub 2006 Apr 21.

DOI:10.1016/j.matbio.2006.03.004

PMID:16631359

Abstract

We analyzed the reactivity of three different commercially available rat monoclonal antibodies raised against mouse laminin-alpha1beta1gamma1 (laminin-111), AL-1, AL-2, and AL-4. Using ELISA assays, Western blot analysis and immunostainings we present refined epitope maps for these three laminin monoclonals. AL-1 reacted, as predicted with laminin alpha1 chain. AL-4 has also been marketed as an alpha1 chain specific probe, but we show here that AL-4 detects mouse laminin beta1 chain, in the distal part of the coiled-coil region. AL-2 was predicted to react with all three chains near the cross-region, but seems to primarily react with laminin beta1 chain.

摘要

我们分析了三种不同的市售抗小鼠层粘连蛋白α1β1γ1（层粘连蛋白-111）的大鼠单克隆抗体AL-1、AL-2和AL-4的反应性。通过酶联免疫吸附测定（ELISA）、蛋白质免疫印迹分析和免疫染色，我们展示了这三种层粘连蛋白单克隆抗体的精确表位图谱。如预期的那样，AL-1与层粘连蛋白α1链发生反应。AL-4也作为α1链特异性探针销售，但我们在此表明，AL-4在卷曲螺旋区域的远端检测到小鼠层粘连蛋白β1链。预计AL-2会在交叉区域附近与所有三条链发生反应，但似乎主要与层粘连蛋白β1链发生反应。