Okamoto Itaru, Seio Kohji, Sekine Mitsuo
Department of Life Science, Tokyo Institute of Technology, Japan.
Bioorg Med Chem Lett. 2006 Jun 15;16(12):3334-6. doi: 10.1016/j.bmcl.2006.02.019. Epub 2006 May 2.
The triplex forming ability of oligonucleotides containing 2'-O-methyl-2-thiouridine (s2Um) and 2-thiothymidine (s2T) was studied. The UV melting experiments revealed that triplex forming oligonucleotides (TFOs) containing both s2Um or s2T stabilized significantly parallel triplexes. The main reason for stabilization of triplexes was due to the stacking effect of the 2-thiocarbonyl group. Moreover, it turned out that these modified TFOs had a high selectivity in recognition of a matched Hoogsteen base from a mismatched one.
研究了含有2'-O-甲基-2-硫代尿苷（s2Um）和2-硫代胸苷（s2T）的寡核苷酸形成三链体的能力。紫外熔解实验表明，同时含有s2Um或s2T的三链体形成寡核苷酸（TFOs）能显著稳定平行三链体。三链体稳定的主要原因是2-硫代羰基基团的堆积效应。此外，结果表明这些修饰的TFOs在识别匹配的Hoogsteen碱基与错配碱基方面具有高选择性。
