Ben Gaied Nouha, Zhao Zhengyun, Gerrard Simon R, Fox Keith R, Brown Tom
School of Chemistry, University of Southampton, Highfield, SO17 1BJ, Southampton, UK.
Chembiochem. 2009 Jul 20;10(11):1839-51. doi: 10.1002/cbic.200900232.
Anthraquinone and pyrene analogues attached to the 3' and/or 5' termini of triplex-forming oligonucleotides (TFOs) by various linkers increased the stability of parallel triple helices. The modifications are simple to synthesize and can be introduced during standard solid-phase oligonucleotide synthesis. Potent triplex stability was achieved by using doubly modified TFOs, which in the most favourable cases gave an increase in melting temperature of 30 degrees C over the unmodified counterparts and maintained their selectivity for the correct target duplex. Such TFOs can produce triplexes with melting temperatures of 40 degrees C at pH 7 even though they do not contain any triplex-stabilizing base analogues. These studies have implications for the design of triplex-forming oligonucleotides for use in biology and nanotechnology.
通过各种连接子连接到三链形成寡核苷酸（TFO）3'和/或5'末端的蒽醌和芘类似物提高了平行三链螺旋的稳定性。这些修饰合成简单，可在标准固相寡核苷酸合成过程中引入。通过使用双重修饰的TFO实现了强大的三链稳定性，在最有利的情况下，与未修饰的对应物相比，其解链温度提高了30℃，并保持了对正确靶双链体的选择性。即使这些TFO不包含任何三链稳定碱基类似物，它们在pH 7时也能产生解链温度为40℃的三链体。这些研究对用于生物学和纳米技术的三链形成寡核苷酸的设计具有启示意义。
