Grünig Christoph R, Duò Angelo, Sieber Thomas N
ETH Zürich, Institute of Integrative Biology (IBZ), Forest Pathology and Dendrology, CH-8092 Zürich, Switzerland.
Fungal Genet Biol. 2006 Jun;43(6):410-21. doi: 10.1016/j.fgb.2006.01.007. Epub 2006 Apr 24.
The genetic structure of the root endophytes Phialocephala fortinii s.l. and Acephala applanata was analyzed in two undisturbed forests. A total of 606 strains isolated from surface-sterilized, fine roots of Picea abies and Vaccinium myrtillus were examined. Two new cryptic species of P. fortinii were recognized and host specialization of A. applanata was confirmed. This species was almost exclusively isolated from roots of P. abies. The index of association did not deviate significantly from zero within any population, suggesting that recombination occurs or had occurred. Significant gene but no genotype flow was detected among study sites for P. fortinii s.l. In contrast, several isolates of A. applanata with both identical multi-locus haplotype and identical ISSR fingerprint were found in both study sites indicating genotype flow or a recent common history.
在两片未受干扰的森林中分析了根部内生真菌福氏拟盘多毛孢（Phialocephala fortinii s.l.）和扁平无头孢（Acephala applanata）的遗传结构。共检测了从挪威云杉（Picea abies）和越橘（Vaccinium myrtillus）经表面消毒的细根中分离出的606个菌株。识别出了福氏拟盘多毛孢的两个新隐种，并证实了扁平无头孢的寄主专一性。该物种几乎仅从挪威云杉的根部分离得到。关联指数在任何种群内均未显著偏离零，这表明重组正在发生或已经发生。在福氏拟盘多毛孢的研究地点之间检测到显著的基因流动，但未检测到基因型流动。相反，在两个研究地点均发现了几株具有相同多位点单倍型和相同ISSR指纹的扁平无头孢分离株，这表明存在基因型流动或近期有共同的历史。
