Iwama Akihiro, Morimoto Taisuke, Tsuji Masahito, Nakamura Koki, Higuchi Naoya, Imaizumi Ichiro, Shibata Naoki, Yamasaki Masahiro, Nakamura Hiroshi
Department of Endodontics and Research Institute of Advanced Oral Science, School Of Dentistry, Aichi-Gakuin University, Nagoya, Japan.
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006 May;101(5):681-6. doi: 10.1016/j.tripleo.2005.06.007. Epub 2005 Oct 12.
The purpose of this study was to investigate the relationship between type 2 diabetes mellitus and anaerobic bacteria detected in infected root canals.
Normal Wistar rats (control) received a standard laboratory diet with water (group A), and GK rats (type 2 diabetes mellitus rats) a normal laboratory diet with water (group B) or a 30% sucrose solution (group C). Chemotaxis assay was conducted on polymorphonuclear leukocytes from the 3 groups, and the numbers of anaerobic bacteria in infected root canals were determined.
In the chemotaxis assay on the polymorphonuclear leukocytes, the chemotactic response of cells in group C was lower than that for groups A and B (P < .01). As to bacteria detected in the root canal, obligate anaerobic bacteria which stained gram negative, were significantly more numerous in group C (P < .01) than in groups A and B.
The metabolic condition produced by type 2 diabetes mellitus in rats might lower the general host resistance against bacterial infection.
本研究旨在调查2型糖尿病与感染根管中检测到的厌氧菌之间的关系。
正常Wistar大鼠（对照组）给予标准实验室饮食并饮水（A组），GK大鼠（2型糖尿病大鼠）给予正常实验室饮食并饮水（B组）或30%蔗糖溶液（C组）。对三组的多形核白细胞进行趋化性测定，并确定感染根管中厌氧菌的数量。
在多形核白细胞的趋化性测定中，C组细胞的趋化反应低于A组和B组（P < 0.01）。关于根管中检测到的细菌，革兰氏阴性专性厌氧菌在C组中的数量显著多于A组和B组（P < 0.01）。
大鼠2型糖尿病产生的代谢状况可能会降低宿主对细菌感染的总体抵抗力。
