Hosseinimehr S J, Nemati A
Department of Medicinal Chemistry, Faculty of Pharmacy, Mazandarn University of Medical Sciences, Sari, Iran.
Br J Radiol. 2006 May;79(941):415-8. doi: 10.1259/bjr/40692384.
The radioprotective effects of hesperidin (HES), a flavonone glucoside, were investigated by using the micronucleus test for anticlastogenic and cell proliferation activity. A single intraperitoneal (ip) administration of hesperidin at doses of 10 mg kg(-1), 20 mg kg(-1), 40 mg kg(-1), 80 mg kg(-1) and 160 mg kg(-1) 45 min prior to gamma irradiation (2 Gy) reduced the frequencies of micronuleated polychromatic erythrocytes (MnPCEs). All five doses of HES significantly reduced the frequencies of MnPCEs and increased PCE/PCE+NCE ratio in mice bone marrow compared with non-drug-treated irradiated control (p<0.0001). There was a drug dose-response effect of HES in reducing MnPCE and increasing the PCE/PCE+NCE ratio in bone marrow cells. The maximum reduction in MnPCE was observed in mice treated with HES at a dose of 80 mg kg(-1). The total MnPCE values were 2.85 fold less in the 80 mg kg(-1) HES group after being exposed to 2 Gy of gamma-rays than those in the respective irradiated control. Our study demonstrates that hesperidin has powerful protective effects on the radiation-induced DNA damage and on the decline in cell proliferation in mouse bone marrow.
通过微核试验研究了黄酮苷橙皮苷（HES）的抗辐射效应及其抗断裂和细胞增殖活性。在γ射线照射（2 Gy）前45分钟，以10 mg kg⁻¹、20 mg kg⁻¹、40 mg kg⁻¹、80 mg kg⁻¹和160 mg kg⁻¹的剂量腹腔注射橙皮苷，可降低微核多色红细胞（MnPCEs）的频率。与未用药的辐射对照组相比，所有五个剂量的HES均显著降低了小鼠骨髓中MnPCEs的频率，并提高了PCE/PCE+NCE比值（p<0.0001）。HES在降低骨髓细胞中MnPCE和提高PCE/PCE+NCE比值方面存在药物剂量反应效应。在接受80 mg kg⁻¹ HES治疗的小鼠中观察到MnPCE的最大降低。在暴露于2 Gy γ射线后，80 mg kg⁻¹ HES组的总MnPCE值比相应的辐射对照组少2.85倍。我们的研究表明，橙皮苷对辐射诱导的DNA损伤和小鼠骨髓细胞增殖下降具有强大的保护作用。
