Ghasemnezhad Targhi Reza, Changizi Vahid, Haddad Farhang, Homayoun Mansour, Soleymanifard Shokouhozaman
Department of Radiology, School of Allied, Tehran University of Medical Sciences, Tehran, Iran.
Department of Biology, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran.
Avicenna J Phytomed. 2016 Nov-Dec;6(6):678-685.
Ionizing radiation produces free radicals which induce DNA damage and cell death. leaf extract (OVLE) is a natural compound and its capability of scavenging free radicals and its antioxidant activity have been demonstrated by many researchers In this study, using micronucleus assay, radioprotective effect of OVLE against clastogenic and cytotoxic effect of gamma irradiation has been investigated in mice bone marrow cells.
OVLE was injected intraperitoneally to the BALB/c mice 1hr prior to gamma irradiation (3Gy) at the doses of 100 and 200 mg/kg. Twenty four hours after irradiation or treatment, animals were killed and smears were prepared from the bone marrow cells. The slides were stained with May Grunwald-Giemsa method and analyzed microscopically. The frequency of micronucleated polychromatic erythrocytes (MnPCEs), micronucleated normochromatic erythrocyte (MnNCEs) and cell proliferation ratio PCE/PCE+NCE (polychromatic erythrocyte/polychromatic erythrocyte + normochromatic erythrocyte) were calculated.
The results showed that gamma irradiation (3Gy) increased the frequency of MnPCEs, MnNCEs and reduced the PCE/PCE+NCE ratio in mice bone marrow compared to the non-irradiated control group (p<0.0001). Injection of OVLE significantly reduced the frequency of MnPCEs (p<0.0001) and MnNCEs (p<0.05) and increased the PCE/PCE+NCE ratio as compared to the irradiated control group (p<0.05).
It seems that OVLE with its antioxidant properties and its capability of scavenging free radicals and reactive oxygen species can reduce the cytotoxic effects of gamma irradiation in mice bone marrow cells.
电离辐射会产生自由基，进而导致DNA损伤和细胞死亡。橄榄叶提取物（OVLE）是一种天然化合物，许多研究人员已证实其具有清除自由基的能力及其抗氧化活性。在本研究中，采用微核试验，研究了OVLE对小鼠骨髓细胞中γ射线致断裂和细胞毒性作用的辐射防护效果。
在γ射线照射（3Gy）前1小时，以100和200mg/kg的剂量对BALB/c小鼠腹腔注射OVLE。照射或处理24小时后，处死动物并制备骨髓细胞涂片。玻片用May Grunwald-Giemsa方法染色并进行显微镜分析。计算微核多染红细胞（MnPCEs）、微核正常红细胞（MnNCEs）的频率以及细胞增殖率PCE/PCE+NCE（多染红细胞/多染红细胞+正常红细胞）。
结果表明，与未照射的对照组相比，γ射线照射（3Gy）增加了小鼠骨髓中MnPCEs、MnNCEs的频率，并降低了PCE/PCE+NCE比值（p<0.0001）。与照射对照组相比，注射OVLE显著降低了MnPCEs的频率（p<0.0001）和MnNCEs的频率（p<0.05），并提高了PCE/PCE+NCE比值（p<0.05）。
OVLE凭借其抗氧化特性以及清除自由基和活性氧的能力，似乎可以降低γ射线照射对小鼠骨髓细胞的细胞毒性作用。
