White J R, Blacksell S D, Lunt R A, Gard G P
CSIRO, Australian Animal Health Laboratory, Geelong, Vic. Australia.
Vet Microbiol. 1991 Nov;29(3-4):237-50. doi: 10.1016/0378-1135(91)90131-x.
The isolation of a monoclonal antibody (1G9/C9) with specificity for the epizootic haemorrhagic disease (EHD) serogroup has enabled the development of a highly sensitive and specific blocking ELISA (B-ELISA) for the detection of serum antibodies to EHD viruses. The assay was sensitive to blocking antibodies present in hyperimmune reference antisera to all six EHD serotypes tested but was unaffected by reference antisera to 19 South African and eight Australian serotypes of the related orbivirus bluetongue virus (BTV). The sensitivity of the EHD B-ELISA exceeded that of an indirect ELISA (I-ELISA) for EHD-specific antibody detection. Serum antibody titres to BTV and EHD in experimental and field sera, including a sentinel herd from which virus isolations were made, were examined in both the BTV and EHD B-ELISA tests. These results showed the B-ELISA was only sensitive to antibodies specific for the homologous serogroup in each case, even where sequential and mixed infections with each virus type occurred.
一种对流行性出血病（EHD）血清群具有特异性的单克隆抗体（1G9/C9）的分离，使得能够开发出一种用于检测EHD病毒血清抗体的高灵敏度和特异性阻断ELISA（B-ELISA）。该检测方法对所有六种测试的EHD血清型的超免疫参考抗血清中存在的阻断抗体敏感，但不受19种南非和8种澳大利亚相关环状病毒蓝舌病病毒（BTV）血清型的参考抗血清的影响。EHD B-ELISA的灵敏度超过了用于检测EHD特异性抗体的间接ELISA（I-ELISA）。在BTV和EHD B-ELISA检测中，检测了实验血清和野外血清（包括从中分离出病毒的一个哨兵牛群）中针对BTV和EHD的血清抗体滴度。这些结果表明，B-ELISA在每种情况下仅对同源血清群的特异性抗体敏感，即使存在每种病毒类型的连续和混合感染。
