Morgan W W, Bermudez J, Chang X Y
Department of Cellular and Structural Biology, University of Texas Health Science Center, San Antonio 78284-7762.
Eur J Pharmacol. 1991 Nov 12;204(3):335-8. doi: 10.1016/0014-2999(91)90861-j.
Primary cultures of rat cerebellar cells were pretreated with various dosages of pentobarbital before the addition of kainic acid or N-methyl-D-aspartic acid in order to assess effects of this drug on the enhancement of cyclic guanosine-3',5'-phosphate (cyclic GMP) mediated by these excitatory agonists. Pentobarbital significantly suppressed kainic acid-induced increases in this cyclic nucleotide at concentrations as low as 5 microM but was only effective in suppressing the N-methyl-D-aspartic acid enhancement at dosages of 100 microM or greater. These data suggest that this barbiturate is a more effective depressant of the stimulatory effects of kainic acid as compared to N-methyl-D-aspartic acid.
在添加海藻酸或N-甲基-D-天冬氨酸之前，先用不同剂量的戊巴比妥预处理大鼠小脑细胞原代培养物，以评估该药物对这些兴奋性激动剂介导的环磷酸鸟苷（cGMP）增强作用的影响。戊巴比妥在低至5 microM的浓度下就能显著抑制海藻酸诱导的这种环核苷酸增加，但仅在100 microM或更高剂量时才能有效抑制N-甲基-D-天冬氨酸的增强作用。这些数据表明，与N-甲基-D-天冬氨酸相比，这种巴比妥类药物对海藻酸刺激作用的抑制效果更有效。
