Couture Simon, Doucet Michèle, Moreau Maxime, Carrier Michel
Département des Sciences Cliniques, Faculté de Médicine Vétérinaire, Université de Montréal, Québec, Canada, J2S 7C6.
Vet Ophthalmol. 2006 May-Jun;9(3):157-64. doi: 10.1111/j.1463-5224.2006.00457.x.
To evaluate the topical effect of various agents, currently used in the treatment of melting ulcers, on gelatinase activity present in the tear film of normal dogs.
Eight normal adult beagles.
Each animal received the following agents: cyclosporine A 1%, N-acetylcysteine 10%, ciprofloxacin 0.3%, ethylenediaminetetraacetic acid (EDTA) 1%, doxycycline 0.001%, polysulfated glycosaminoglycans (PSGAG) 5%, autoserum, and artificial tears during a 48-h period following a Latin square design. Tear samples were collected with micro-capillary pipettes following a corneal surface irrigation of each eye with sterile saline on four different occasions. Basal and total gelatinase activities were evaluated by optical density after processing in a commercial gelatinase activity assay. From the optical density ratio, a semi-quantitative measure of gelatinase activity was obtained. Basal and total activities were measured in all samples.
The lowest total gelatinase activity, representing a percent decline in the enzyme activity although not significant, was observed 1 h after the last treatment in seven out of the eight ophthalmic agents; EDTA (68%), ciprofloxacin (76%), cyclosporine A (68%), doxycycline (47%), artificial tears (26%), PSGAG (25%), and N-acetylcysteine (20%). However, only the reduction observed with EDTA 6 h after the last treatment was significantly lower compared to the reduction observed with the artificial tears.
This study indicated that only EDTA was able to significantly reduce the gelatinase activity in a persistent manner in the tear film of normal canine eyes. Further studies will be required to evaluate the effect of EDTA under ulcerative conditions and to more accurately ascertain the potential in vivo effect of the other agents.
评估目前用于治疗溶解性溃疡的各种药物对正常犬泪膜中明胶酶活性的局部作用。
八只正常成年比格犬。
每只动物按照拉丁方设计在48小时内接受以下药物:1%环孢素A、10%N - 乙酰半胱氨酸、0.3%环丙沙星、1%乙二胺四乙酸(EDTA)、0.001%强力霉素、5%多硫酸化糖胺聚糖(PSGAG)、自体血清和人工泪液。在每次用无菌盐水冲洗每只眼睛的角膜表面后的四个不同时间点,用微量毛细管移液器收集泪液样本。在商业明胶酶活性测定中处理后,通过光密度评估基础和总明胶酶活性。从光密度比中获得明胶酶活性的半定量测量值。在所有样本中测量基础和总活性。
在八种眼科药物中的七种药物末次治疗后1小时观察到最低的总明胶酶活性,尽管酶活性下降百分比不显著;EDTA(68%)、环丙沙星(76%)、环孢素A(68%)、强力霉素(47%)、人工泪液(26%)、PSGAG(25%)和N - 乙酰半胱氨酸(20%)。然而,与人工泪液相比,仅在末次治疗后6小时观察到的EDTA导致的降低显著更低。
本研究表明,只有EDTA能够持续显著降低正常犬眼泪膜中的明胶酶活性。需要进一步研究来评估EDTA在溃疡性条件下的作用,并更准确地确定其他药物的潜在体内作用。