De Sarro G, Nisticó G
Institute of Pharmacology, Faculty of Medicine, University of Catanzaro, Italy.
Int J Neurosci. 1991 Jul;59(1-3):67-79. doi: 10.3109/00207459108985450.
Human recombinant interleukin-2 and rat recombinant IL-2 microinjected into the locus coeruleus of rats, induced typical dose-dependent behavioural sedation and/or sleep and electrocortical synchronization. During sleep induced by this lymphokine a dose-dependent increase in total voltage power (0.25-16 Hz) as well as in the 0.25-3, 3-6 and 6-9 Hz frequency bands was observed. The behavioural and electrocortical effects of interleukin-2 were blocked in animals pretreated with anti-IL-2 monoclonal antibodies and with naloxone, whereas they were still evident in rats pretreated with yohimbine. In addition, the behavioural and electrocortical slow-wave sleep effects observed after the administration of interleukin-2 into the locus coeruleus were reduced significantly or antagonized completely by a previous pretreatment with pertussis toxin, forskolin, dibutyryl-cyclic-AMP and 8-bromo-cyclic-AMP. These results are consistent with the hypothesis that the behavioural and electrocortical changes of this lymphokine are mediated at locus coeruleus level via a guanine regulatory Gi protein coupling IL-2 specific receptors to the adenylate cyclase system.
将人重组白细胞介素 -2 和大鼠重组白细胞介素 -2 微量注射到大鼠的蓝斑中，可诱导出典型的剂量依赖性行为镇静和/或睡眠以及皮层电同步。在这种淋巴因子诱导的睡眠期间，观察到总电压功率（0.25 - 16 Hz）以及 0.25 - 3、3 - 6 和 6 - 9 Hz 频段出现剂量依赖性增加。白细胞介素 -2 的行为和皮层电效应在用抗白细胞介素 -2 单克隆抗体和纳洛酮预处理的动物中被阻断，而在用育亨宾预处理的大鼠中仍然明显。此外，在蓝斑中注射白细胞介素 -2 后观察到的行为和皮层电慢波睡眠效应，在用百日咳毒素、福斯可林、二丁酰环磷腺苷和 8 - 溴环磷腺苷预先预处理后显著降低或完全拮抗。这些结果与以下假设一致，即这种淋巴因子的行为和皮层电变化在蓝斑水平通过将白细胞介素 -2 特异性受体与腺苷酸环化酶系统偶联的鸟嘌呤调节 Gi 蛋白介导。
