Park Joo Hun, Kim Young Sun, Lee Hye Lim, Shim Jin Young, Lee Keu Sung, Oh Yoon Jung, Shin Seung Soo, Choi Young Hwa, Park Kwang Joo, Park Rae Woong, Hwang Sung Chul
Department of Pulmonary and Critical Care Medicine, Ajou University School of Medicine, Suwon, Korea.
Respirology. 2006 May;11(3):269-75. doi: 10.1111/j.1440-1843.2006.00849.x.
Peroxiredoxins (Prxs) have been implicated in regulating many cellular processes including cell proliferation, differentiation and apoptosis. However, the pathophysiological significance of Prx proteins, especially in lung disease, has not been defined. Therefore, the authors investigated the distribution and expression of various Prx isoforms in lung cancer and compared this with normal lung from human and mouse.
Patients diagnosed with lung cancer who underwent surgery at Ajou Medical Center were enrolled. Expression of Prxs, thioredoxin and thioredoxin reductase was analysed by proteomic techniques. Immunohistochemistry was performed to localize Prx proteins.
Immunohistochemical staining showed that the isoforms of Prx I, II, III and V were predominantly expressed in bronchial and alveolar epithelium as well as in alveolar macrophages of the normal mouse lung. The isoforms I, III and thioredoxin were overexpressed in lung cancer tissues compared with normal lungs. There was also an increased amount of oxidized form of Prx I and a putative truncated form of Prx III in lung cancer samples when analysed on two-dimensional electrophoresis. In addition, a 40-kDa intermediate MW protein band and high MW bands of over 20 kDa, recognized by anti-Prx (a-Prx) I antibody, were present in tissue extracts of lung cancer patients on one-dimensional electrophoresis.
The upregulation of Prx I, Prx III and thioredoxin in lung cancer tissue may represent an attempt by tumour cells to adjust to the microenvironment in a manner that is advantageous to survival and proliferation.
过氧化物还原酶（Prxs）参与调控许多细胞过程，包括细胞增殖、分化和凋亡。然而，Prx蛋白的病理生理学意义，尤其是在肺部疾病中的意义，尚未明确。因此，作者研究了各种Prx亚型在肺癌中的分布和表达，并将其与人和小鼠的正常肺组织进行比较。
纳入在阿朱医疗中心接受手术的肺癌患者。通过蛋白质组学技术分析Prxs、硫氧还蛋白和硫氧还蛋白还原酶的表达。进行免疫组织化学以定位Prx蛋白。
免疫组织化学染色显示，Prx I、II、III和V亚型主要表达于正常小鼠肺的支气管和肺泡上皮以及肺泡巨噬细胞中。与正常肺相比，Prx I、III亚型和硫氧还蛋白在肺癌组织中过表达。二维电泳分析显示，肺癌样本中Prx I的氧化形式和Prx III的一种假定截短形式的量也增加。此外，一维电泳显示，肺癌患者组织提取物中存在一条40 kDa的中等分子量蛋白带和多条大于20 kDa的高分子量带，可被抗Prx（a-Prx）I抗体识别。
肺癌组织中Prx I、Prx III和硫氧还蛋白的上调可能代表肿瘤细胞试图以有利于生存和增殖的方式适应微环境。
