Pari Leelavinothan, Gnanasoundari Muthurangam
Department of Biochemistry, Faculty of Science, Annamalai University, Annamalainagar - 608002, Tamilnadu, India.
Basic Clin Pharmacol Toxicol. 2006 May;98(5):456-61. doi: 10.1111/j.1742-7843.2006.pto_351.x.
Naringenin is a naturally occurring citrus flavanone, which has been reported to have a wide range of pharmacological properties. The present work was carried out to evaluate the effect of naringenin on antioxidant and lipid peroxidation status in liver of oxytetracycline-intoxicated rats. Intraperitonial administration of oxytetracycline 200 mg/kg for 15 days resulted a significant elevation in serum hepatospecific markers such as aspartate transaminase, alanine transaminase, alkaline phosphatase, lactate dehydrogenase, and bilirubin and the levels of lipid peroxidation markers (thiobarbituric acid reactive substances (TBARS) and lipid hydroperoxides) in liver. Oxytetracycline also caused a significant reduction in the activities of superoxide dismutase, catalase, glutathione peroxidase, reduced glutathione (GSH), vitamin C and vitamin E in liver. Oral administration of naringenin (50 mg/kg b.w.t.) with oxytetracycline significantly decreased the activities of serum aspartate transaminase, alanine transaminase, alkaline phosphatase, lactate dehydrogenase and the levels of bilirubin along with significant decrease in the levels of lipid peroxidation markers in the liver. In addition, naringenin significantly increased the activities of superoxide dismutase, catalase and GSH peroxidase as well as the level of GSH, vitamin C and vitamin E in liver of the oxytetracycline-treated rats. Our results demonstrate that naringenin exhibited antioxidant property and decrease the lipid peroxidation against oxytetracycline-induced oxidative stress in liver.
柚皮素是一种天然存在的柑橘类黄酮，据报道具有广泛的药理特性。本研究旨在评估柚皮素对土霉素中毒大鼠肝脏抗氧化和脂质过氧化状态的影响。腹腔注射200mg/kg土霉素，持续15天，导致血清肝特异性标志物如天冬氨酸转氨酶、丙氨酸转氨酶、碱性磷酸酶、乳酸脱氢酶和胆红素显著升高，同时肝脏中脂质过氧化标志物（硫代巴比妥酸反应性物质（TBARS）和脂质氢过氧化物）水平也显著升高。土霉素还导致肝脏中超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶、还原型谷胱甘肽（GSH）、维生素C和维生素E的活性显著降低。与土霉素一起口服柚皮素（50mg/kg体重）可显著降低血清天冬氨酸转氨酶、丙氨酸转氨酶、碱性磷酸酶、乳酸脱氢酶的活性以及胆红素水平，同时肝脏中脂质过氧化标志物水平也显著降低。此外，柚皮素显著提高了土霉素处理大鼠肝脏中超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶的活性以及GSH、维生素C和维生素E的水平。我们的结果表明，柚皮素具有抗氧化特性，并能减轻土霉素诱导的肝脏氧化应激导致的脂质过氧化。
