[基质金属蛋白酶-9和金属蛋白酶组织抑制剂-1 mRNA在子宫内膜异位症中的表达]

[Expressions of matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 mRNA in endometriosis].

作者信息

Li Yan, Lang Jing-he

机构信息

Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Science, Beijing 100730, China.

出版信息

Zhonghua Fu Chan Ke Za Zhi. 2006 Jan;41(1):30-3.

PMID:16635325

Abstract

OBJECTIVE

To investigate mRNA expression of matrix metalloproteinase (MMP-9) and tissue inhibitor of metalloproteinase (TIMP-1) in ectopic endometriosis tissue and uterine endometrium from women with and without endometriosis.

METHODS

Thirty-eight women with endometriosis (Revised American Fertility Society classification, RAFSI-IV) were selected as study group. Thirty-eight specimens of ovarian endometrioma (ovarian chocolate cysts, OCC), 16 red peritoneal endometriotic lesions (RPL), and 35 matched eutopic endometrium (Eu) were collected from them simultaneously at the time of surgery. Twenty specimens of endometrium from reproductive women undergoing laparoscopic surgery without endometriosis were obtained as control group. The mRNA expressions of MMP-9 and TIMP-1 were detected by reverse transcription polymerase chain reaction (RT-PCR).

RESULTS

Expression of TIMP-1 mRNA was detected in all samples. The level from endometriosis patients and control group was similar (2.31 +/- 1.21, 2.40 +/- 0.89). However, ectopic endometrium expressed significantly fewer TIMP-1 mRNA (OCC 1.67 +/- 0.79, RPL 1.45 +/- 0.68) compared with eutopic endometrium from both endometriosis and endometriosis-free patients (P < 0.05). The positive expression rate of MMP-9 mRNA was not distinctively different among all samples. The density of MMP-9 mRNA expression in endometrium (0.49 +/- 0.28) from endometriosis patients was similar to that in ectopic endometriosis (OCC 0.46 +/- 0.22, RPL 0.33 +/- 0.12), but was significantly higher compared with endometrium (0.29 +/- 0.12) without endometriosis (P < 0.05).

CONCLUSIONS

An increase of MMP-9 mRNA expression of eutopic endometrium with endometriosis might enhance the endometrial implantation ability, thus facilitate the ectopic implantation of endometrium. Ectopic lesions express significantly less TIMP-1 mRNA, indicating they have increased invasive ability, which might facilitate the development of endometriosis.

摘要

目的

研究基质金属蛋白酶（MMP - 9）和金属蛋白酶组织抑制剂（TIMP - 1）在有或无子宫内膜异位症女性的异位子宫内膜组织及子宫内膜中的mRNA表达情况。

方法

选取38例子宫内膜异位症患者（修订后的美国生育协会分类，RAFSI - IV）作为研究组。在手术时同时采集她们的38个卵巢子宫内膜异位囊肿标本（卵巢巧克力囊肿，OCC）、16个红色腹膜子宫内膜异位病灶标本（RPL）以及35个配对的在位内膜标本（Eu）。选取20例接受腹腔镜手术且无子宫内膜异位症的育龄期女性的子宫内膜标本作为对照组。采用逆转录聚合酶链反应（RT - PCR）检测MMP - 9和TIMP - 1的mRNA表达。

结果

所有样本均检测到TIMP - 1 mRNA的表达。子宫内膜异位症患者组与对照组的TIMP - 1 mRNA水平相似（分别为2.31±1.21和2.40±0.89）。然而，与子宫内膜异位症患者和非子宫内膜异位症患者的在位内膜相比，异位内膜中TIMP - 1 mRNA的表达明显减少（OCC为1.67±0.79，RPL为1.45±0.68）（P < 0.05）。所有样本中MMP - 9 mRNA的阳性表达率无明显差异。子宫内膜异位症患者的在位内膜中MMP - 9 mRNA的表达密度（0.49±0.28）与异位子宫内膜（OCC为0.46±0.22，RPL为0.33±0.12）相似，但与无子宫内膜异位症的子宫内膜（0.29±0.12）相比明显更高（P < 0.05）。