Qiu Fang, Gao Xue-mei, Luo Guo-lin, Yang Kai-xuan, Wang He, Liu Hui, Wen Yi
Gynecology, West China Second Hospital, Sichuan University, Chengdu 610041, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2006 Jan;37(1):118-22.
To investigate the expressions of matrix metalloproteinase -2, -3 (MMP-2,-3) and tissue inhibitor of metalloproteinase-2 (TIMP-2) in internal uterine endometriosis tissue and endometrium from women with and without endometriosis throughout the menstrual cycle.
Immunohistochemical staining of tissues was performed to study the expressions and locations of MMP-2, MMP-3, TIMP-2 between women with and without endometriosis. The real-time PCR technique was applied to detected the mRNA expressions of MMP-2, MMP-3 and TIMP-2.
We found that ectopic endometrium from patients with endometriosis expressed higher levels of MMP-2 and MMP-3 and lower levers of TIMP-2 than normal controls did (P < 0.05). MMP-2 and MMP-3 were detected strongly in both stromal and epithelial cells of ectopic endometrium from patients with endometriosis, when mostly detected in the epithelial cells in the control group. All eutopic and ectopic endometrium samples from women with and without endometriosis throughout the menstrual cycle showed similar expressions of MMP-2, MMP-3 and TIMP-2. Quantitative expressions of MMP-2 mRNA, MMP-3 mRNA and TIMP-2 mRNA were significantly lower in eutopic endometrium from controls compared with ectopic endometrium from patients with endometriosis (P < 0.05). Eutopic endometrium from controls in the proliferative phase showed significantly increased expressions of MMP-2 mRNA compared with that in the secretory phase (P < 0.05).
The results suggest that ectopic endometrium with adenomyosis has more invasiveness and is prone to peritoneal implantation maybe to involved in the high expressions of MMP-2 and MMP-3 and the less expressions of TIMP-2 than endometrium from women without adenomyosis does.
研究基质金属蛋白酶-2、-3（MMP-2、-3）和金属蛋白酶组织抑制剂-2（TIMP-2）在子宫内膜异位症患者子宫内异位内膜组织及整个月经周期中有或无子宫内膜异位症女性的子宫内膜中的表达情况。
采用组织免疫组化染色法研究有或无子宫内膜异位症女性之间MMP-2、MMP-3、TIMP-2的表达及定位。应用实时荧光定量PCR技术检测MMP-2、MMP-3和TIMP-2的mRNA表达。
我们发现，子宫内膜异位症患者的异位内膜中MMP-2和MMP-3的表达水平高于正常对照组，而TIMP-2的表达水平低于正常对照组（P<0.05）。在子宫内膜异位症患者的异位内膜基质细胞和上皮细胞中均强烈检测到MMP-2和MMP-3，而在对照组中主要在上皮细胞中检测到。在整个月经周期中，有或无子宫内膜异位症女性的所有在位和异位内膜样本中MMP-2、MMP-3和TIMP-2的表达相似。与子宫内膜异位症患者的异位内膜相比，对照组在位内膜中MMP-2 mRNA、MMP-3 mRNA和TIMP-2 mRNA的定量表达显著降低（P<0.05）。与分泌期相比，增殖期对照组在位内膜中MMP-2 mRNA的表达显著增加（P<0.05）。
结果表明，与无子宫腺肌病女性的子宫内膜相比，子宫腺肌病异位内膜具有更强的侵袭性，易于腹膜种植，这可能与MMP-2和MMP-3的高表达及TIMP-2的低表达有关。
