[人源新基因NM23-H1B在卵巢肿瘤中的mRNA表达研究]

[Study on mRNA expression of the human novel gene NM23-H1B in ovarian tumor].

作者信息

Li Wen, Liu Yan, Jin Zhi-jun, Feng You-ji, Huang La-mei, Chen Jing

机构信息

Department of Obstetrics and Gynecology, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China.

出版信息

Zhonghua Fu Chan Ke Za Zhi. 2006 Jan;41(1):48-51.

PMID:16635329

Abstract

OBJECTIVE

To study the expression of the human novel gene NM23-H1B in ovarian cancer.

METHODS

Forty-eight samples from patients with ovarian tumor at different clinical stages and 8 from normal ovaries were examined for NM23-H1B mRNA expression by using RT-PCR, northern blot and in situ hybridization.

RESULTS

All samples expressed NM23-H1B mRNA through RT-PCR, while the level of expression in ovarian tumor was higher than that of normal ovary. The level of expression in early stage (stage I and II) cancer was higher than in advanced (stage III and IV) cancer. The results of northern blot showed that NM23-H1B was over expressed in ovarian cancer while low expressed in normal ovary or low malignant potential (LMP) ovarian cancer. In early stage carcinoma, the expression level was related with the differentiation of tumor cell. Well-differentiated cancer expressed NM23-H1B mRNA at comparatively higher level. The result of in situ hybridization showed that positive expression rate of NM23-H1B mRNA in ovarian cancer (100%, 40/40) was significantly higher than that in normal ovary (0/8) or LMP ovarian cancer (2/8).

CONCLUSION

The novel gene NM23-H1B is related to ovarian cancer.

摘要

目的

研究人类新基因NM23-H1B在卵巢癌中的表达。

方法

采用逆转录聚合酶链反应（RT-PCR）、Northern印迹法和原位杂交技术，检测48例不同临床分期的卵巢肿瘤患者的样本以及8例正常卵巢组织样本中NM23-H1B mRNA的表达情况。

结果

RT-PCR结果显示所有样本均表达NM23-H1B mRNA，卵巢肿瘤中的表达水平高于正常卵巢。早期（Ⅰ期和Ⅱ期）癌的表达水平高于晚期（Ⅲ期和Ⅳ期）癌。Northern印迹法结果显示，NM23-H1B在卵巢癌中高表达，而在正常卵巢或低恶性潜能（LMP）卵巢癌中低表达。在早期癌中，表达水平与肿瘤细胞的分化有关。高分化癌中NM23-H1B mRNA的表达水平相对较高。原位杂交结果显示，卵巢癌中NM23-H1B mRNA的阳性表达率（100%，40/40）显著高于正常卵巢（0/8）或LMP卵巢癌（2/8）。