一种碳水化合物结合蛋白，半乳糖凝集素-1，可促进成体神经干细胞的增殖。
A carbohydrate-binding protein, Galectin-1, promotes proliferation of adult neural stem cells.
作者信息
Sakaguchi Masanori, Shingo Tetsuro, Shimazaki Takuya, Okano Hirotaka James, Shiwa Mieko, Ishibashi Satoru, Oguro Hideyuki, Ninomiya Mikiko, Kadoya Toshihiko, Horie Hidenori, Shibuya Akira, Mizusawa Hidehiro, Poirier Françoise, Nakauchi Hiromitsu, Sawamoto Kazunobu, Okano Hideyuki
机构信息
Department of Physiology, Keio University School of Medicine, Tokyo 160-8582, Japan.
出版信息
Proc Natl Acad Sci U S A. 2006 May 2;103(18):7112-7. doi: 10.1073/pnas.0508793103. Epub 2006 Apr 24.
Abstract
In the subventricular zone of the adult mammalian forebrain, neural stem cells (NSCs) reside and proliferate to generate young neurons. We screened factors that promoted the proliferation of NSCs in vitro by a recently developed proteomics technique, the ProteinChip system. In this screen, we identified a soluble carbohydrate-binding protein, Galectin-1, as a candidate. We show herein that Galectin-1 is expressed in a subset of slowly dividing subventricular zone astrocytes, which includes the NSCs. Based on results from intraventricular infusion experiments and phenotypic analyses of knockout mice, we demonstrate that Galectin-1 is an endogenous factor that promotes the proliferation of NSCs in the adult brain.
摘要
在成年哺乳动物前脑的脑室下区，神经干细胞（NSCs）存在并增殖以产生年轻的神经元。我们通过最近开发的蛋白质组学技术——蛋白质芯片系统，筛选了在体外促进神经干细胞增殖的因子。在这次筛选中，我们鉴定出一种可溶性碳水化合物结合蛋白——半乳糖凝集素-1作为候选因子。我们在此表明，半乳糖凝集素-1在包括神经干细胞在内的缓慢分裂的脑室下区星形胶质细胞亚群中表达。基于脑室内注射实验结果和基因敲除小鼠的表型分析，我们证明半乳糖凝集素-1是一种促进成年大脑中神经干细胞增殖的内源性因子。
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