Guarda E, Corbalan R, Lavandero S, Martínez S, Ocaranza M P, Sapag-Hagar M, Casanegra P, Valenzuela C
Departamento de Enfermedades Cardiovasculares, Pontificia Universidad Católica de Chile, Santiago de Chile.
Rev Med Chil. 1990 Jun;118(6):617-21.
Severe decompensated chronic heart failure is associated to increased levels of circulating catecholamines and decreased density of myocardial beta-adrenergic receptors. In 14 patients with stable, class II-III heart failure we studied circulating lymphocytes to determine the number of beta adrenergic receptors, the dissociation constant of 3H dihydroalprenolol (kd) and the intracellular content of cyclic AMP (AMPc). Results (mean +/- SEM) were compared to those obtained in 10 healthy controls. The number of beta receptors was significantly decreased (105 +/- 16 vs 185 +/- 24, fmol/mg of membrane protein, p less than 0.01). No differences were found in Kd (1.65 +/- 0.2 vs. 1.36 +/- 0.28 nm) nor the level of AMPc (7.9 +/- 2.1 vs 7.1 +/- 2.9 pmol/mg protein), respectively. The decreased number of beta adrenergic receptors in the circulating lymphocytes may be related to the increased level of circulating catecholamines that have been shown to be present during exercise in these patients.
严重失代偿性慢性心力衰竭与循环儿茶酚胺水平升高及心肌β-肾上腺素能受体密度降低有关。在14例稳定的II-III级心力衰竭患者中，我们研究了循环淋巴细胞，以确定β-肾上腺素能受体的数量、3H-二氢阿普洛尔的解离常数（kd）和细胞内环磷酸腺苷（AMPc）的含量。将结果（平均值±标准误）与10名健康对照者的结果进行比较。β受体数量显著减少（105±16对185±24，fmol/毫克膜蛋白，p<0.01）。kd（1.65±0.2对1.36±0.28纳米）和AMPc水平（7.9±2.1对7.1±2.9 pmol/毫克蛋白）分别未发现差异。循环淋巴细胞中β-肾上腺素能受体数量减少可能与这些患者运动时循环儿茶酚胺水平升高有关。
