一种人源抗Jkb单克隆抗体。
A human anti-Jkb monoclonal antibody.
作者信息
Lecointre-Coatmelec M, Bourel D, Ferrette J, Genetet B
机构信息
Centre Régional de Transfusion Sanguine, Rennes, France.
出版信息
Vox Sang. 1991;61(4):255-7. doi: 10.1111/j.1423-0410.1991.tb00956.x.
Abstract
Peripheral blood mononuclear cells of an immunized patient were transformed with Epstein-Barr virus and then fused with P3X63Ag8 mouse myeloma cells by polyethylene glycol. After the cloning, a hybridoma cell line secreting specific anti-Jkb monoclonal antibody was isolated. The antibody was produced in supernatant form and tested for its use as a blood grouping reagent.
摘要
将免疫患者的外周血单个核细胞用爱泼斯坦-巴尔病毒转化，然后通过聚乙二醇与P3X63Ag8小鼠骨髓瘤细胞融合。克隆后，分离出分泌特异性抗Jkb单克隆抗体的杂交瘤细胞系。该抗体以上清液形式产生，并作为血型鉴定试剂进行测试。
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