Krüger Carina, Hultberg Anna, Marcotte Harold, Hermans Pim, Bezemer Sandra, Frenken Leon G J, Hammarström Lennart
Division of Clinical Immunology, Karolinska Institutet at Karolinska University Hospital, Stockholm, 141 86, Sweden.
Appl Microbiol Biotechnol. 2006 Oct;72(4):732-7. doi: 10.1007/s00253-006-0347-0. Epub 2006 Apr 25.
Streptococcus mutans is the main cause of dental caries. We evaluated the therapeutic effect of variable regions of a llama heavy chain antibody fragments directed against S. mutans named S36-VHH (S for Streptococcus) alone or fused with glucose oxidase (GOx) from Aspergillus niger. Western blot analysis and ELISA revealed binding of the S36-VHH to the streptococcal antigen I/II adhesin molecule of S. mutans serotype C. In a rat-desalivated caries model, daily administration of S36-VHH significantly reduced the development of smooth surface caries. No additional therapeutic effect of GOx was observed. Our results suggest that llama VHH antibodies may be a potential benefit as prophylaxis against dental caries.
变形链球菌是龋齿的主要病因。我们评估了一种针对变形链球菌的羊驼重链抗体片段可变区（命名为S36-VHH，S代表链球菌）单独使用或与黑曲霉葡萄糖氧化酶（GOx）融合后的治疗效果。蛋白质免疫印迹分析和酶联免疫吸附测定显示，S36-VHH与变形链球菌血清型C的链球菌抗原I/II粘附分子结合。在大鼠去唾液龋齿模型中，每日给予S36-VHH可显著减少光滑面龋齿的发生。未观察到GOx的额外治疗效果。我们的结果表明，羊驼VHH抗体可能作为预防龋齿的潜在有益物质。
