Kawabe Akira, Nasuda Shuhei
Laboratory of Plant Genetics, Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake-cho, 606-8502, Sakyo-ku, Kyoto, Japan.
Genetica. 2006 Mar;126(3):335-42. doi: 10.1007/s10709-005-0718-y.
The chromosomal localizations of repetitive DNA clusters (ribosomal DNA and centromere satellites) were analyzed by fluorescent in situ hybridization in five strains of Arabidopsis halleri ssp. gemmifera. All five A. gemmifera strains have three chromosome pairs with 45S (5.8S-16S-26S) rDNA loci, and one pair with both 5S and 45S rDNA loci. These localizations are different from that of A. thaliana. Very unusually, there are three families of centromeric satellite DNAs (pAa, pAge1, and pAge2), and they showed polymorphism among the five strains studied. Overall, we found four different centromere satellite compositions. A plant from Fumuro was heterozygous for the chromosome specificities of centromere satellite families, possibly due to a reciprocal translocation involving centromere regions. Changes of centromeric satellite repeats appear to be rapid and frequent events in the history of A. gemmifera, and seem to occur by exchanging clusters as units.
通过荧光原位杂交技术，对五株深山南芥亚种细叶山芥中的重复DNA簇（核糖体DNA和着丝粒卫星DNA）的染色体定位进行了分析。所有五株细叶山芥都有三对染色体带有45S（5.8S - 16S - 26S）rDNA位点，还有一对染色体同时带有5S和45S rDNA位点。这些定位与拟南芥不同。非常特别的是，存在三个着丝粒卫星DNA家族（pAa、pAge1和pAge2），并且在研究的五株菌株中表现出多态性。总体而言，我们发现了四种不同的着丝粒卫星组成。来自文室的一株植物在着丝粒卫星家族的染色体特异性方面是杂合的，这可能是由于涉及着丝粒区域的相互易位所致。着丝粒卫星重复序列的变化在细叶山芥的历史中似乎是快速且频繁发生的事件，并且似乎是以簇作为单位进行交换而发生的。
