Faraji M, Okhovvat S M
College of Agriculture, University of Tehran, 31587-11167 Karaj, Iran.
Commun Agric Appl Biol Sci. 2005;70(3):315-8.
In greenhouse, susceptibility of 6 cultivars of bean (b1, green bean cv. contender, b2, Pinto b. cv. Cos-16, b3, green b. Sun Ray, b4, white b. cv. Daneshkadeh, b5, red b. cv. Naz, b6, red b. cv. Goli) evaluated to 7 isolates of 2 species of Fusarium (6 isolates of F. oxysporum and 1 isolate of F. solani from Varamin, Iran) in 4 pots (as replications) in factoriel experiment. The seedlings of each bean cultivars soaked in a suspension of the fungi (106 spores/ml.) for 5 minutes and transplanted into the pots contained sterilized soil, for control, the roots soaked in distilled water. After 35 days of inoculating and transplanting in greenhouse conditions (with 18-26 degrees C and 12 h light & 12 h dark) the percentage of necrotic roots and stems of the seedlings affected to the isolates of the fungi, also height and weight of them in different treatments datermined and calculated. The results showed that, the average of necrotic percent of the cultivars of bean seedlings grouped in 3 category (b1, b2, b3, b4 with 25.15% in group a and b5, red bean cv. Naz with 16.29% in group b and b6 red bean cv. Goli with 10.02% in group c) based on cluster analysis. But by Dunken method the infection set on 5 groups as respectively b4, 28,72% (a), b2, 25.83% (ab), b1 & b3, 23,18% & 22.84% (b), b5, 16.29% (c) and b6, 10.02% (d). So, red bean cv. Goli was more resistant and white bean cv. Daneshkadeh was more susceptible than of another cultivars, but averse the effect of the fungi on growth factors were different.
在温室中,通过析因试验,以4盆(作为重复)对6个菜豆品种(b1,绿豆品种竞争者;b2,平托豆品种Cos - 16;b3,绿豆品种Sun Ray;b4,白豆品种Daneshkadeh;b5,红豆品种Naz;b6,红豆品种Goli)对镰刀菌属2个种的7个分离株(6个尖孢镰刀菌分离株和1个来自伊朗瓦拉明的茄病镰刀菌分离株)的敏感性进行了评估。将每个菜豆品种的幼苗在真菌悬浮液(106个孢子/毫升)中浸泡5分钟,然后移植到装有灭菌土壤的花盆中,作为对照,将根部浸泡在蒸馏水中。在温室条件下(温度为18 - 26摄氏度,光照12小时,黑暗12小时)接种和移植35天后,测定并计算了受真菌分离株影响的幼苗坏死根和茎的百分比,以及它们在不同处理下的高度和重量。结果表明,根据聚类分析,菜豆品种幼苗坏死百分比的平均值分为3类(b1、b2、b3、b4在a组,坏死率为25.15%;b5,红豆品种Naz在b组,坏死率为16.29%;b6,红豆品种Goli在c组,坏死率为10.02%)。但通过邓肯法,感染分为5组,分别为b4,28.72%(a);b2,25.83%(ab);b1和b3,23.18%和22.84%(b);b5,16.29%(c);b6,10.02%(d)。因此,红豆品种Goli比其他品种更抗病,白豆品种Daneshkadeh比其他品种更感病,但真菌对生长因子的影响不同。