Petrova R R, Mezhevinkina L M, Fesenko E E
Biofizika. 2006 Mar-Apr;51(2):310-5.
An experimental model of differentiated mouse embryonic stem cells with retractive activity similar to that of cardiomyocytes without preliminary formation of embryoid bodies was obtained. The basic factor that induced in vitro embryonic cell differentiation into cardial type is the recombinant cytokine LIF under prolonged cultivation. The positive reaction of the cells with retractive activity to isoproterenol indicates the presence of the beta-adrenergic receptor activity characteristic only for terminal differentiated mammalian cardiomyocytes.
获得了一种分化的小鼠胚胎干细胞实验模型，其具有类似于心肌细胞的收缩活性，且未预先形成胚状体。在长时间培养下，诱导体外胚胎细胞分化为心脏型的基本因素是重组细胞因子白血病抑制因子（LIF）。具有收缩活性的细胞对异丙肾上腺素的阳性反应表明存在仅终末分化的哺乳动物心肌细胞特有的β-肾上腺素能受体活性。
