Nieuwenhuizen Lauren, Khalil Mohd K, Venkatesh Naik, Othman Nor Hayati
Department of Pathology, Universiti Sains Malaysia, Kubang Kerian, Malaysia.
Anal Quant Cytol Histol. 2006 Apr;28(2):87-96.
To determine the ideal histochemical stain to differentiate between non-neoplastic and neoplastic endocervix and endometrium.
A total of 90 cases representing nonneoplastic cervix, non-neoplastic endometrium, endocervical adenocarcinoma and endometrial adenocarcinoma were stained with toluidine blue (TB); methylene blue (MB); mucicarmine (MUC); periodic acid-Schiff before and after diastase digestion (PAS, PAS-D); Alcian blue, pH 2.5 (AB); and periodic acid-Schiff after Alcian blue, pH 2.5 (PAB). Cases were blinded and randomly divided between two pathologists for evaluation of the staining and the staining distribution of the glandular epithelium by means of a 36-color scheme.
The majority of non-neoplastic endocervix samples stained blue with MB (57%), fuchsia with MUC (70%), magenta with PAS (77%) and PAS-D (73%) and dark turquoise with AB (70%). The majority of non-neoplastic endometrium samples stained slate blue with TB (60%) and pink with PAS-D (53.3%). There is statistical difference (p < 0.05) in the color of the epithelium and secretions between the non-neoplastic cervix and endometrium. The malignant glands of endocervical origin could be differentiated significantly (p = 0.043) from non-neoplastic endocervical epithelium by MUC. The epithelium of the non-neoplastic endometrium is significantly differentiated from malignant endometrium using TB (p = 0.015) and MB (p = 0.038). Endocervical carcinoma could be significantly differentiated from endometrial carcinoma by MB. The staining in endocervical adenocarcinoma and endometrial carcinoma was predominantly present in both apical and cytoplasmic locations compared to their non-neoplastic counterparts (endocervix, p = 0.003; endometrium, p = 0.049).
This study showed that a panel of histochemical stains could differentiate glandular cells of endocervical epithelium from endometrium.
确定用于鉴别非肿瘤性和肿瘤性宫颈内膜及子宫内膜的理想组织化学染色方法。
选取90例代表非肿瘤性宫颈、非肿瘤性子宫内膜、宫颈腺癌和子宫内膜腺癌的病例，分别用甲苯胺蓝（TB）、亚甲蓝（MB）、黏液卡红（MUC）、淀粉酶消化前后的过碘酸希夫染色（PAS、PAS-D）、pH 2.5的阿尔辛蓝（AB）以及pH 2.5的阿尔辛蓝后过碘酸希夫染色（PAB）进行染色。病例进行盲法处理，并随机分配给两位病理学家，通过36色方案评估染色情况及腺上皮的染色分布。
大多数非肿瘤性宫颈内膜样本用MB染色呈蓝色（57%），用MUC染色呈紫红色（70%），用PAS和PAS-D染色呈品红色（77%和73%），用AB染色呈深绿松石色（70%）。大多数非肿瘤性子宫内膜样本用TB染色呈石板蓝色（60%），用PAS-D染色呈粉红色（53.3%）。非肿瘤性宫颈内膜和子宫内膜的上皮及分泌物颜色存在统计学差异（p < 0.05）。MUC可将宫颈来源的恶性腺体与非肿瘤性宫颈内膜上皮显著区分（p = 0.043）。使用TB（p = 0.015）和MB（p = 0.038）可将非肿瘤性子宫内膜上皮与恶性子宫内膜显著区分。MB可将宫颈癌与子宫内膜癌显著区分。与非肿瘤对应物（宫颈内膜，p = 0.003；子宫内膜，p = 0.049）相比，宫颈腺癌和子宫内膜癌的染色主要出现在顶端和细胞质部位。
本研究表明，一组组织化学染色可区分宫颈内膜上皮和子宫内膜的腺细胞。
