Toda T, Sadi A M, Egawa H, Atari E, Qureshi B, Nagai Y
Pathology Section, Diagnostic Laboratory, University Hospital, University of the Ryukyus, Okinawa, Japan.
Histopathology. 1998 Mar;32(3):257-63. doi: 10.1046/j.1365-2559.1998.00346.x.
Endocervical and endometrial tissues were stained with four lectins to determine the difference in staining pattern between non-neoplastic and neoplastic conditions of these tissues.
The lectins used were Ulex europaeus agglutinin (UEA), Dolicho biflorus agglutinin (DBA). Concanavalin A (Con A), and Phaseolus vulgaris agglutinin (PHA). Endocervical tissues included normal endocervical glands, microglandular hyperplasia, minimal deviation adenocarcinoma and endocervical adenocarcinoma, well to poorly differentiated types. Endometrial tissues were collected from normal endometrium, simple glandular hyperplasia, complex hyperplasia, atypical hyperplasia and adenocarcinoma grades 1-3. Non-neoplastic and neoplastic endocervical and endometrial glandular epithelium showed positive reaction for UEA, Con A and PHA. Non-neoplastic glands showed mild to moderate intensity and apical and/polar type of staining pattern for all lectins. Endocervical adenocarcinoma including minimal deviation adenocarcinoma (MDC) and adenocarcinoma well to moderately differentiated type showed diffuse cytoplasmic type of staining pattern for all lectins, but poorly differentiated adenocarcinoma of endocervix showed only a stromal pattern for all lectins. Endometrial hyperplasia and adenocarcinoma grades 1-3 showed positive reaction for all lectins except for DBA. The staining pattern of endometrial hyperplasia was variable, but adenocarcinoma grades 1-3 showed diffuse type.
Intensity and staining patterns of lectins are helpful in distinguishing between endocervical and endometrial non-neoplastic and neoplastic lesions. Intense positive reaction of MDC, especially for Con A and PHA, can differentiate this lesion from normal endocervical glands. The stromal type of staining pattern of poorly differentiated endocervical adenocarcinoma can also have diagnostic significance. Negative reactions of DBA lectin for endometrial adenocarcinoma can be used for differentiating it from endocervical adenocarcinoma.
用四种凝集素对子宫颈内膜和子宫内膜组织进行染色，以确定这些组织在非肿瘤性和肿瘤性状态下染色模式的差异。
所用凝集素为欧洲荆豆凝集素（UEA）、双花扁豆凝集素（DBA）、刀豆球蛋白A（Con A）和菜豆凝集素（PHA）。子宫颈内膜组织包括正常子宫颈腺、微小腺体增生、微小偏离腺癌和子宫颈腺癌，涵盖高分化至低分化类型。子宫内膜组织取自正常子宫内膜、单纯性腺体增生、复杂性增生、不典型增生及1 - 3级腺癌。非肿瘤性和肿瘤性子宫颈内膜及子宫内膜腺上皮对UEA、Con A和PHA呈阳性反应。非肿瘤性腺体对所有凝集素均显示轻度至中度强度以及顶端和/或极性染色模式。子宫颈腺癌包括微小偏离腺癌（MDC）以及高分化至中分化型腺癌对所有凝集素均显示弥漫性胞质染色模式，但子宫颈低分化腺癌对所有凝集素仅显示间质染色模式。子宫内膜增生及1 - 3级腺癌对除DBA外的所有凝集素呈阳性反应。子宫内膜增生的染色模式多变，但1 - 3级腺癌显示弥漫型。
凝集素的强度和染色模式有助于区分子宫颈内膜和子宫内膜的非肿瘤性及肿瘤性病变。MDC的强烈阳性反应，尤其是对Con A和PHA的反应，可将此病变与正常子宫颈腺区分开来。子宫颈低分化腺癌的间质型染色模式也具有诊断意义。DBA凝集素对子宫内膜腺癌的阴性反应可用于将其与子宫颈腺癌区分开来。
