脂质囊泡中细胞、蛋白质和微珠的可控微流控封装。

Controlled microfluidic encapsulation of cells, proteins, and microbeads in lipid vesicles.

作者信息

Tan Yung-Chieh, Hettiarachchi Kanaka, Siu Maria, Pan Yen-Ru, Lee Abraham Phillip

机构信息

University of California Irvine, Department of Biomedical Engineering, Irvine, California 92697, USA.

出版信息

J Am Chem Soc. 2006 May 3;128(17):5656-8. doi: 10.1021/ja056641h.

DOI:10.1021/ja056641h

PMID:16637631

Abstract

Cells have been encapsulated inside lipid vesicles by using a new microfluidic lipid vesicle formulation technique. Lipid vesicles are formulated within minutes without using toxic lipid solvents. The encapsulation efficiency inside the vesicles is controlled by the microfluidic flows. Green fluorescent proteins (GFP), carcinoma cells, and bead encapsulated vesicles have mean diameters of 27.2 mum, 62.4 mum, and 55.9 mum, respectively. The variations of vesicle sizes are approximately 20% for the GFP and cell encapsulated vesicles and approximately 10% for the bead encapsulated vesicles.

摘要

通过一种新的微流控脂质囊泡制备技术，细胞已被包裹在脂质囊泡内部。脂质囊泡在数分钟内即可制备完成，无需使用有毒的脂质溶剂。囊泡内部的包封效率由微流控流动控制。绿色荧光蛋白（GFP）、癌细胞和包裹有珠子的囊泡的平均直径分别为27.2微米、62.4微米和55.9微米。对于包裹有GFP和细胞的囊泡，囊泡大小的变化约为20%，对于包裹有珠子的囊泡，囊泡大小的变化约为10%。