Manthey John A
Citrus and Subtropical Products Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Winter Haven, Florida 33881, USA.
J Agric Food Chem. 2006 May 3;54(9):3215-8. doi: 10.1021/jf053134a.
Preliminary studies have shown that orange peel polymethoxylated flavones (PMFs) exhibit beneficial biological properties in animals. These properties have increased the demands for these compounds as candidate nutraceuticals and specialty food ingredients. Orange oil residues are a likely commercial source of the PMFs, and a rapid, solvent-free method for the analysis of the PMFs in orange oil residues has been developed based on Fourier transform infrared (FTIR) spectroscopy. The intensities of the FTIR vibrations of the phenyl ring nu(C=C) stretch at 1515 cm(-1) of the PMFs can be used, relative to the intensity of the carbonyl stretch at 1733 cm(-1) of the non-PMF orange oil residue components, to measure PMF content. Excellent correlations for the ratios of the intensities of these vibrations and the total PMF content were observed irrespective of the source, viscosity, and presence of particulate material. The detection limit by this method is approximately 0.1% PMF.
初步研究表明，橙皮多甲氧基黄酮（PMFs）在动物体内具有有益的生物学特性。这些特性增加了对这些化合物作为候选营养保健品和特色食品成分的需求。橙油残渣是PMFs的一个可能的商业来源，并且基于傅里叶变换红外（FTIR）光谱法开发了一种快速、无溶剂的方法来分析橙油残渣中的PMFs。相对于非PMF橙油残渣成分在1733 cm(-1)处羰基伸缩振动的强度，PMFs在1515 cm(-1)处苯环ν(C=C)伸缩振动的FTIR振动强度可用于测量PMF含量。无论来源、粘度和颗粒物质的存在情况如何，均观察到这些振动强度的比值与总PMF含量之间具有良好的相关性。该方法的检测限约为0.1% PMF。
