Chien Wei-Jyun, Saputri Dinar S, Lin Hung-Yu
Department of Applied Chemistry Chaoyang University of Technology Taichung, Taiwan.
Curr Res Food Sci. 2022 Jan 27;5:278-287. doi: 10.1016/j.crfs.2022.01.013. eCollection 2022.
As the highest yield crop worldwide, citrus peels that possess bioactive compounds were discarded as a futile by-product. Ultrasonication with environmentally friendly solvent (50% ethanol and ddHO) were used in the present study to extract flavonoids from Hayata peels with extraction period and fruit maturity as other variables. DPPH scavenging activity was investigated. Qualitative flavonoid content analysis was done by UV/Vis and FTIR-ATR spectra. Quantification of flavonoid using LC-MS/MS found that solvent type, fruit maturity, and ultrasonication period significantly affect the extracted flavonoid yield (p < 0.05). Extraction using 50% ethanol showed a higher yield than ddHO. Flavonoid content was also higher in unripe than ripe samples. Nobiletin, tangeretin, and rutin were dominant among the identified compounds in all sample treatments. Flavonoid content in Hayata extract was found to negatively correlate to DPPH scavenging activity, which needs further research to identify other bioactivities of these flavonoids.
作为全球产量最高的作物，富含生物活性化合物的柑橘皮被当作无用的副产品丢弃。本研究采用环保型溶剂（50%乙醇和双蒸水）超声提取法，以提取时间和果实成熟度为变量，从早田氏枳壳果皮中提取黄酮类化合物。研究了其对DPPH自由基的清除活性。通过紫外/可见光谱和傅里叶变换红外光谱衰减全反射法对黄酮类化合物进行定性含量分析。使用液相色谱-串联质谱法定量分析黄酮类化合物发现，溶剂类型、果实成熟度和超声提取时间显著影响黄酮类化合物的提取产率（p < 0.05）。使用50%乙醇提取的产率高于双蒸水。未成熟样品中的黄酮类化合物含量也高于成熟样品。在所有样品处理中，川陈皮素、橘红素和芦丁是鉴定出的主要化合物。早田氏枳壳提取物中的黄酮类化合物含量与DPPH自由基清除活性呈负相关，这需要进一步研究以确定这些黄酮类化合物的其他生物活性。
